PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species

Two PCR assays have been developed to detect Aspergillus carbonarius and Aspergillus ochraceus, considered the main sources of ochratoxin A (OTA) contaminating commodities, particularly grapes, coffee and derivatives, in warm climates. The species specific primers have been designed on the basis of...

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Bibliographic Details
Published in:International journal of food microbiology 2005-10, Vol.104 (2), p.207-214
Main Authors: Patino, B, Gonzalez-Salgado, A, Gonzalez-Jaen, M.T, Vazquez, C
Format: Article
Language:English
Subjects:
Aspergillus - classification
Aspergillus - isolation & purification
Aspergillus - metabolism
Aspergillus alutaceus
Aspergillus carbonarius
Aspergillus ochraceus
Aspergillus ochraceus - classification
Aspergillus ochraceus - isolation & purification
Aspergillus ochraceus - metabolism
Base Sequence
Biological and medical sciences
coffee (beverage)
Detection
DNA, Ribosomal - analysis
food contamination
Food Contamination - analysis
Food industries
Food Microbiology
food pathogens
Fundamental and applied biological sciences. Psychology
Gene Amplification
General aspects
grapes
ITS
Methods of analysis, processing and quality control, regulation, standards
microbial detection
Molecular Sequence Data
nucleotide sequences
Ochratoxin A
Ochratoxins - isolation & purification
Ochratoxins - metabolism
PCR
Phylogeny
polymerase chain reaction
Polymerase Chain Reaction - methods
secondary metabolites
Sequence Alignment
Species Specificity
toxigenic strains
Vitaceae
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Description
Summary:Two PCR assays have been developed to detect Aspergillus carbonarius and Aspergillus ochraceus, considered the main sources of ochratoxin A (OTA) contaminating commodities, particularly grapes, coffee and derivatives, in warm climates. The species specific primers have been designed on the basis of ITS (internal transcribed spacers of rDNA units) sequence comparisons obtained from Aspergillus strains and have been tested in a number of strains from different origins and hosts. These PCR assays, based on multi-copy sequences, are highly sensitive and specific and represent a good tool for an early detection of OTA-producing Aspergillus species and to prevent OTA entering the food chain.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2005.02.011