Determination of relationships among non-toxigenic Vibrio cholerae O1 biotype El Tor strains from housekeeping gene sequences and ribotype patterns

Sequencing of three housekeeping genes, mdh, dnaE and recA, and ribotyping for seven non-toxigenic Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three to...

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Bibliographic Details
Published in:Research in microbiology 2009-01, Vol.160 (1), p.57-62
Main Authors: Mohapatra, Saswat S., Ramachandran, Dhanya, Mantri, Chinmay K., Colwell, Rita R., Singh, Durg V.
Format: Article
Language:English
Subjects:
Bacteriology
Base Sequence
Biological and medical sciences
DNA Polymerase III - genetics
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Variation
Housekeeping genes
Malate Dehydrogenase - genetics
Marine
Microbiology
Miscellaneous
Molecular Sequence Data
Multigene Family
Phylogeny
Rec A Recombinases - genetics
Recombination, Genetic
Ribotyping
Sequence Analysis, DNA
Sequencing
Vibrio cholerae
Vibrio cholerae O1 - classification
Vibrio cholerae O1 - genetics
Vibrio cholerae O1 - pathogenicity
Virulence
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Summary:Sequencing of three housekeeping genes, mdh, dnaE and recA, and ribotyping for seven non-toxigenic Vibrio cholerae O1 strains isolated from different geographic sources indicate a phylogenetic relationship among the strains. Results of MLST and ribotyping indicate a clear difference between three toxigenic strains (N16961, O395, and 569B) and three non-toxigenic strains from India (GS1, GS2, and GW87) and one Guam strain (X392), the latter of which were similar in both MLST and ribotyping, while two other non-toxigenic strains from the USA and India (2740-80 and OR69) appeared to be more closely related to toxigenic strains than to non-toxigenic strains, although this was not supported by ribotyping. These results provide clues to the emergence of toxigenic strains from a non-toxigenic progenitor by acquisition of virulence gene clusters. Results of split decomposition analysis suggest that widespread recombination occurs among the three housekeeping genes and that recombination plays an important role in the emergence of toxigenic strains of V. cholerae O1.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2008.10.008