The stability of antimycobacterial drugs in media used for drug susceptibility testing

The emergence of drug-resistant tuberculosis and disease caused by nontuberculous mycobacteria has increased the need for accurate drug susceptibility testing of mycobacteria. The stability of the tested drugs in relevant test media have been understudied. We assessed the stability of isoniazid, rif...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2018-12, Vol.92 (4), p.305-308
Main Authors: Schoutrop, Esther L.M., Brouwer, Michelle A.E., Jenniskens, Josien C.A., Ferro, Beatriz E., Mouton, Johan W., Aarnoutse, Rob E., van Ingen, Jakko
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Clarithromycin - pharmacology
Culture Media, Conditioned
Dose-Response Relationship, Drug
Drug Resistance, Bacterial
Drug Stability
Drug susceptibility testing
Humans
Microbial Sensitivity Tests - methods
Mycobacteria
Mycobacterium - drug effects
Rifampicin, clarithromycin
Rifampin - pharmacology
Stability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The emergence of drug-resistant tuberculosis and disease caused by nontuberculous mycobacteria has increased the need for accurate drug susceptibility testing of mycobacteria. The stability of the tested drugs in relevant test media have been understudied. We assessed the stability of isoniazid, rifampicin, clarithromycin, linezolid and amikacin in Middlebrook 7H9 medium and that of clarithromycin, amikacin and cefoxitin in the cation-adjusted Mueller Hinton broth. We used ultra-performance liquid chromatography (UPLC) methods for rifampicin and isoniazid and a microbiological assay for rifampicin, clarithromycin, amikacin, cefoxitin and linezolid. Rifampicin and isoniazid concentrations in Middlebrook 7H9 medium had decreased by 92% and 54% after 7 days. The microbiological assay revealed decreases in drug concentration of ≥75% (rifampicin, clarithromycin, cefoxitin) and 60% (linezolid) after 14 days. With the exception of amikacin, all antimycobacterial drugs were unstable during 14 days of incubation in the preferred media for DST. Drug stability may influence minimum inhibitory concentration measurements.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2018.06.015