Vacuum Ultraviolet Laser Desorption/Ionization Mass Spectrometry Imaging of Single Cells with Submicron Craters

Mass spectrometry imaging (MSI) is a crucial label-free method to distinguish the localization patterns in single cells. MALDI-TOF MS and ToF-SIMS are now bearing the responsibility. However, MALDI-TOF MS is limited to micron spatial resolution and ToF-SIMS suffers from severe molecular fragmentatio...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2018-08, Vol.90 (16), p.10009-10015
Main Authors: Wang, Jia, Wang, Zhaoying, Liu, Feng, Cai, Lesi, Pan, Jian-bin, Li, Zhanping, Zhang, Sichun, Chen, Hong-Yuan, Zhang, Xinrong, Mo, Yuxiang
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Craters
Desorption
Fluorescence
Fragmentation
Ionization
Ions
Mass spectrometry
Molecular chemistry
Spatial resolution
Spectroscopy
Ultraviolet lasers
Ultraviolet radiation
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Summary:Mass spectrometry imaging (MSI) is a crucial label-free method to distinguish the localization patterns in single cells. MALDI-TOF MS and ToF-SIMS are now bearing the responsibility. However, MALDI-TOF MS is limited to micron spatial resolution and ToF-SIMS suffers from severe molecular fragmentation. Here, we proposed a new MSI methodology of vacuum ultraviolet laser desorption/ionization (VUVDI) with high spatial resolution, achieving higher ion yields and less fragmentation compared with ToF-SIMS at submicron level. The fluorescence image and mass spectrum of VUVDI were obtained simultaneously. In addition, the adjustable laser fluence acquired selective detection for different molecular and fragmental ions, thus realizing molecular identification. Furthermore, MSIs of single cells with submicron craters were presented. These results suggest VUVDI is a potential mass spectrometry method that provides a soft ionization source and submicron spatial resolution for molecular analysis in life science.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.8b02478