Binding and hydrolysis properties of engineered cellobiohydrolases and endoglucanases

• Published in: Bioresource technology 2018-11, Vol.267, p.235-241
• Main Authors: Lu, Xianqin, Feng, Xiaoting, Li, Xuezhi, Zhao, Jian
• Format: Article
• Language: English
• Subjects: Adsorption; Cellobiohydrolases; Endoglucanases; Engineered cellulases; Hydrolysis
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2018.06.047

•The engineered cellulases performed well during hydrolysis.•Fusion of T. r-CBMV27E,P30D,Link1 to cellulase enhanced the binding and hydrolysis efficiency of cellulase.•Binding conformations of polysaccharide on CBMs hypothetically resulted in different functions of CBMs. Because cellulase was the main enzyme used in bioconversion of lignocellulose, it was a valid way to reduce the hydrolysis cost by increasing the adsorption and hydrolysis efficiency of cellulase. In this study, modified cellobiohydrolases (CBHs) and endoglucanases (EGs) were constructed. Two engineered cellulases CBH-TrCBMV27E,P30D,Link1 and EG-TrCBMV27E,P30D,Link1 well-performed during hydrolysis. Compared to wild-type enzymes, EG-TrCBMV27E,P30D,Link1 had relatively less adsorption ability to lignin and greater affinity to cellulose, especially Avicel. However, for CBH-TrCBMV27E,P30D,Link1, the hydrolysis manner was changed and in favor to hydrolysis process, although the adsorption properties were unexpected. It suggested that various binding conformations of polysaccharide on CBMs hypothetically resulted in different functions of CBMs, including binding ability, processive and digestive properties on fiber surface. Fusion of T. r-CBMV27E,P30D,Link1 to cellulase, both CBH and EG, gave the destruction ability of enzyme and increased the accessible surface of substrate to cellulase, enhanced the adsorption and hydrolysis efficiency of cellulase.