Novel electrospun fibers with incorporated commensal bacteria for potential preventive treatment of the diabetic foot

• Published in: Nanomedicine (London, England) England), 2018-07, Vol.13 (13), p.1583-1594
• Main Authors: Kure i, Manja, Rijavec, Toma, Hribernik, Silvo, Lapanje, Aleš, Kleinschek, Karin S, Maver, Uroš
• Format: Article
• Language: English
• Subjects: Amputation; Bacteria; Biocompatible Materials - administration & dosage; Biocompatible Materials - chemistry; Biofilms; Carboxymethylcellulose Sodium - administration & dosage; Carboxymethylcellulose Sodium - chemistry; cell electrospinning; commensal bacteria; controlled release; Diabetes; diabetic foot; Diabetic Foot - drug therapy; Diabetic Foot - microbiology; Diabetic Foot - pathology; Foot diseases; Humans; Leg ulcers; Mass spectrometry; Microbiota; Microbiota - drug effects; Microorganisms; Microscopy, Electron, Scanning; Nanofibers - administration & dosage; Nanofibers - chemistry; Nanofibers - microbiology; Nosocomial infections; Pathogens; Peptides; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Polyvinyl alcohol; Prevention; prophylactic/preventive treatment; release kinetics; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcus epidermidis - chemistry; Staphylococcus epidermidis - growth & development; Staphylococcus epidermidis - ultrastructure; Symbiosis
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm-2018-0014

A novel electrospun biocompatible nanofibrous material loaded with commensal bacteria for potential preventive treatment of the diabetic foot was developed. Two biocompatible polymers (carboxymethylcellulose and polyethylene oxide) were combined with a bacterium isolate from the skin located between the toes of a healthy adult (identified using a matrix-assisted laser desorption/ionization mass spectrometry-based method as a strain of ). Higher bacteria loads in the material were assured through their encapsulation in polyethylenimine. The nanofibrous material was characterized using scanning electron microscopy, zeta-potential measurements and through evaluation of cell growth and viability. nanometer formation was confirmed using scanning electron microscopy, while the zeta-potential measurements revealed successful bacteria encapsulation. Viable and sufficiently growing cells were confirmed prior and after their incorporation. The prepared materials were proven suitable to deliver viable commensal bacteria in a comparable share to the Staphylococcaceae in the foot microbiome making this approach promising for preventive diabetic foot treatment.