Detection of ST1702 Escherichia coli blaNDM-5 and blaCMY-42 genes positive isolates from a Northern Italian hospital

We describe two multi drug-resistant (MDR) carbapenemase-producing Escherichia coli clinical isolates from an acute hospital in Milan. Both strains, isolated from a surgical wound sample and a surveillance rectal swab respectively, were positive for a blaNDM-type gene by Xpert Carba-R test. The whol...

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Bibliographic Details
Published in:The New microbiologica 2018-07, Vol.41 (3), p.230-231
Main Authors: Piazza, Aurora, Comandatore, Francesco, Romeri, Francesca, Pagani, Cristina, Mattioni Marchetti, Vittoria, Brilli, Matteo, Panelli, Simona, Migliavacca, Roberta, Ridolfo, Annalisa, Olivieri, Pietro, Gismondo, Maria Rita, Bandi, Claudio, Rimoldi, Sara Giordana
Format: Article
Language:English
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Summary:We describe two multi drug-resistant (MDR) carbapenemase-producing Escherichia coli clinical isolates from an acute hospital in Milan. Both strains, isolated from a surgical wound sample and a surveillance rectal swab respectively, were positive for a blaNDM-type gene by Xpert Carba-R test. The whole-genome sequence characterization disclosed several resistance determinants: blaNDM-5, blaCMY-42, blaTEM-198, rmtB, mphA. The two isolates belonged to phylogenetic group A, sequence type (ST) 1702 and serotype O89:H9. PCR-based replicon typing and conjugation assay demonstrated an IncI1 plasmid localization for both blaNDM-5 and blaCMY-42 genes. This is the first report of a ST1702 NDM-5 and CMY-42- producing E. coli clone in Italy.
ISSN:1121-7138