Boswellic acids and glucosamine show synergistic effect in preclinical anti-inflammatory study in rats

• Published in: Bioorganic & medicinal chemistry letters 2007-07, Vol.17 (13), p.3706-3711
• Main Authors: Singh, Surjeet, Khajuria, Anamika, Taneja, Subhash Chandra, Khajuria, Ravi Kant, Singh, Jaswant, Qazi, Ghulam Nabi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Arthritis - drug therapy; Biological and medical sciences; Bones, joints and connective tissue. Antiinflammatory agents; Carrageenan - pharmacology; Chemistry, Pharmaceutical - methods; Drug Design; Drug Evaluation, Preclinical; Drug Synergism; Edema; Glucosamine - chemistry; Glucosamine - metabolism; Magnetic Resonance Spectroscopy; Medical sciences; Models, Chemical; Mycobacterium - metabolism; Pharmacology. Drug treatments; Rats; Triterpenes - chemistry
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2007.04.034

Leukotriene inhibiting boswellic acids and glucosamine individually are known for their anti-arthritic potential in preclinical and clinical studies. A combination of both displayed significant synergism in chronic inflammation, whereas in acute inflammation it was almost insignificant. The present study revealed the synergistic effect of boswellic acid mixture (BA) and glucosamine for anti-inflammatory and anti-arthritic activities in rats. Two studies were conducted, that is, acute anti-inflammatory by carrageenan edema and chronic anti-arthritic by Mycobacterium-induced developing arthritis. Five groups of animals were included in each of the study: the vehicle control, positive control (ibuprofen 100 mg/kg), boswellic acids (250 mg/kg), glucosamine (250 mg/kg) and a combination of boswellic acids (125 mg/kg) and glucosamine (125 mg/kg). BA when administered at 250 mg/kg in rats, carrageenan-induced paw edema and Mycobacterium-induced developing arthritis were significantly inhibited. In comparison to boswellic acids, glucosamine when administered at 250 mg/kg showed a mild effect in carrageenan-induced edema and moderate inhibition of paw swelling against developing arthritis. Although the combination of boswellic acids and glucosamine did not affect the acute inflammation to a greater extent yet a significant anti-arthritic activity was observed in rats. In conclusion, a synergistic effect was observed in chronic inflammatory conditions when two chemical entities were administered in combination in preclinical study.