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Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis
The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific v...
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Published in: | Journal of phytopathology 2009-02, Vol.157 (2), p.94-100 |
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description | The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific variability within isolates of an Armillaria gallica population sampled from a Quercus spp. stand located in Gravina in Puglia, southern Italy. UPGMA cluster analysis of RAPD profiles generated by decamer primers grouped the isolates in subclusters demonstrating relatively low intraspecific genetic variability. Moreover, RAPD pattern analysis yielded clusters which did not correspond to the groups discriminated by vegetative compatibility tests performed by a previous investigation on the same population. The findings of this research pose the question of whether somatic incompatibility, which involves an undefined number of genes and alleles per gene, might still be considered an effective tool for the epidemiological studies of A. gallica, whereas molecular analyses are more useful for assessing genomic variation within the species. |
doi_str_mv | 10.1111/j.1439-0434.2008.01456.x |
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The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific variability within isolates of an Armillaria gallica population sampled from a Quercus spp. stand located in Gravina in Puglia, southern Italy. UPGMA cluster analysis of RAPD profiles generated by decamer primers grouped the isolates in subclusters demonstrating relatively low intraspecific genetic variability. Moreover, RAPD pattern analysis yielded clusters which did not correspond to the groups discriminated by vegetative compatibility tests performed by a previous investigation on the same population. The findings of this research pose the question of whether somatic incompatibility, which involves an undefined number of genes and alleles per gene, might still be considered an effective tool for the epidemiological studies of A. gallica, whereas molecular analyses are more useful for assessing genomic variation within the species.</description><identifier>ISSN: 0931-1785</identifier><identifier>EISSN: 1439-0434</identifier><identifier>DOI: 10.1111/j.1439-0434.2008.01456.x</identifier><identifier>CODEN: JPHYEB</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Armillaria ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; genetic variation ; oak decline ; Phytopathology. Animal pests. Plant and forest protection ; Quercus ; root rot</subject><ispartof>Journal of phytopathology, 2009-02, Vol.157 (2), p.94-100</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Verlag, Berlin</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3576-61a393b2ae945421db57f78bf1ae3a67da8569a68b61f5c57dceeeb5a82fbcdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21030251$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Gatto, Angela</creatorcontrib><creatorcontrib>Sicoli, Giovanni</creatorcontrib><creatorcontrib>Luisi, Nicola</creatorcontrib><title>Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis</title><title>Journal of phytopathology</title><description>The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific variability within isolates of an Armillaria gallica population sampled from a Quercus spp. stand located in Gravina in Puglia, southern Italy. UPGMA cluster analysis of RAPD profiles generated by decamer primers grouped the isolates in subclusters demonstrating relatively low intraspecific genetic variability. Moreover, RAPD pattern analysis yielded clusters which did not correspond to the groups discriminated by vegetative compatibility tests performed by a previous investigation on the same population. The findings of this research pose the question of whether somatic incompatibility, which involves an undefined number of genes and alleles per gene, might still be considered an effective tool for the epidemiological studies of A. gallica, whereas molecular analyses are more useful for assessing genomic variation within the species.</description><subject>Armillaria</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>genetic variation</subject><subject>oak decline</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Quercus</subject><subject>root rot</subject><issn>0931-1785</issn><issn>1439-0434</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkMGO0zAQhi0EEmXhHXyBW4Idx3Fy4BC6bLdoBdUKVG7WxHHAxU26npRt3h6HrnrGsjSW_H8zo48QylnK43m_S3kuqoTlIk8zxsqU8VwW6ekZWVw-npMFqwRPuCrlS_IKccdYxgRjC3Jc2d6OztBr98cGdONEH934y_UUeroewbtYN8Ph6GF0Q0-Hjt4MweJI67B33kNwQH-C984AXeMQYxYpIK0RbbwtbSZ6X2-uk83yntY9-AkdviYvOvBo3zzVK_L95tO35W1y93W1XtZ3iRFSFUnBQVSiycBWucwz3jZSdapsOg5WQKFaKGVRQVE2Be-kkao11tpGQpl1jWmNuCLvzn0PYXg4xq313qGxce3eDkfUGVO5KBWLwfIcNGFADLbTh-D2ECbNmZ49652edepZp54963-e9Smib59mABrwXYDeOLzwGY-iM8lj7sM59-i8nf67v_68uZ1fkU_OvMPRni48hN-6UEJJvf2y0j8-lttMqFxvxV8ze6D5</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Gatto, Angela</creator><creator>Sicoli, Giovanni</creator><creator>Luisi, Nicola</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200902</creationdate><title>Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis</title><author>Gatto, Angela ; Sicoli, Giovanni ; Luisi, Nicola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3576-61a393b2ae945421db57f78bf1ae3a67da8569a68b61f5c57dceeeb5a82fbcdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Armillaria</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>genetic variation</topic><topic>oak decline</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Quercus</topic><topic>root rot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gatto, Angela</creatorcontrib><creatorcontrib>Sicoli, Giovanni</creatorcontrib><creatorcontrib>Luisi, Nicola</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gatto, Angela</au><au>Sicoli, Giovanni</au><au>Luisi, Nicola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis</atitle><jtitle>Journal of phytopathology</jtitle><date>2009-02</date><risdate>2009</risdate><volume>157</volume><issue>2</issue><spage>94</spage><epage>100</epage><pages>94-100</pages><issn>0931-1785</issn><eissn>1439-0434</eissn><coden>JPHYEB</coden><abstract>The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific variability within isolates of an Armillaria gallica population sampled from a Quercus spp. stand located in Gravina in Puglia, southern Italy. UPGMA cluster analysis of RAPD profiles generated by decamer primers grouped the isolates in subclusters demonstrating relatively low intraspecific genetic variability. Moreover, RAPD pattern analysis yielded clusters which did not correspond to the groups discriminated by vegetative compatibility tests performed by a previous investigation on the same population. The findings of this research pose the question of whether somatic incompatibility, which involves an undefined number of genes and alleles per gene, might still be considered an effective tool for the epidemiological studies of A. gallica, whereas molecular analyses are more useful for assessing genomic variation within the species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1439-0434.2008.01456.x</doi><tpages>7</tpages></addata></record> |
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source | Wiley-Blackwell Read & Publish Collection |
subjects | Armillaria Biological and medical sciences Fundamental and applied biological sciences. Psychology Fungal plant pathogens genetic variation oak decline Phytopathology. Animal pests. Plant and forest protection Quercus root rot |
title | Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis |
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