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Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis

The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific v...

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Published in:Journal of phytopathology 2009-02, Vol.157 (2), p.94-100
Main Authors: Gatto, Angela, Sicoli, Giovanni, Luisi, Nicola
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Language:English
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Sicoli, Giovanni
Luisi, Nicola
description The genus Armillaria includes harmful fungal pathogens that cause root rot and wood decay in a broad range of host plants throughout the world. The aim of this study was to detect, by means of Random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) markers, the level of intraspecific variability within isolates of an Armillaria gallica population sampled from a Quercus spp. stand located in Gravina in Puglia, southern Italy. UPGMA cluster analysis of RAPD profiles generated by decamer primers grouped the isolates in subclusters demonstrating relatively low intraspecific genetic variability. Moreover, RAPD pattern analysis yielded clusters which did not correspond to the groups discriminated by vegetative compatibility tests performed by a previous investigation on the same population. The findings of this research pose the question of whether somatic incompatibility, which involves an undefined number of genes and alleles per gene, might still be considered an effective tool for the epidemiological studies of A. gallica, whereas molecular analyses are more useful for assessing genomic variation within the species.
doi_str_mv 10.1111/j.1439-0434.2008.01456.x
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source Wiley-Blackwell Read & Publish Collection
subjects Armillaria
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
genetic variation
oak decline
Phytopathology. Animal pests. Plant and forest protection
Quercus
root rot
title Genetic Diversity within an Italian Population of Forest Armillaria gallica Isolates as Assessed by RAPD-PCR Analysis
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