In Vitro and In Vivo Evaluation of Ferric-Hyaluronate Implants for Delivery of Amikacin Sulfate to the Tarsocrural Joint of Horses

• Published in: Veterinary surgery 2009-06, Vol.38 (4), p.498-505
• Main Authors: CRIBB, NICOLA C, BOURÉ, LUDOVIC P, BRAD HANNA, W.J, AKENS, MARGARETE K, MATTSON, SHAWN E, MONTEITH, GABRIELLE J, WEESE, J. SCOTT
• Format: Article
• Language: English
• Subjects: Absorbable Implants - veterinary; Amikacin - administration & dosage; Amikacin - pharmacokinetics; amikacin sulfate; Animals; Antibiotics; antimicrobial agents; blood composition; cartilage; cell biology; Clinical outcomes; Drug Delivery Systems; drug implants; ferric-hyaluronate implants; horse diseases; Horses; hyaluronic acid; Hyaluronic Acid - chemistry; in vitro studies; in vivo studies; Injections, Intra-Articular - veterinary; Iron - chemistry; joint diseases; Joints; joints (animal); pharmacokinetics; Staphylococcus aureus; synovial fluid; tarsocrural joint; Tarsus, Animal; toxicity; Transplants & implants; veterinary drugs; Veterinary medicine; viability
• ISSN: 0161-3499; 1532-950X
• DOI: 10.1111/j.1532-950X.2009.00518.x

To assess the antimicrobial elution characteristics, toxicity, and antimicrobial activity of amikacin-impregnated ferric-hyaluronate implants (AI-FeHAI) for amikacin delivery to the tarsocrural joint of horses. Experimental study. AI-FeHAI implants, equine cartilage, and synovium, and horses (n=6). In vitro study: Five AI-FeHAI were placed in saline solution with daily replacement until implant degradation. Eluent was tested for amikacin concentration and bioactivity. Synovial and cartilage explants were incubated in the presence or absence of AI-FeHAI for 72 hours and subsequently assessed for morphology, viability, and composition. Synovial explants were incubated with Staphylococcus aureus in the presence or absence of AI-FeHAI. Spent medium was cultured daily and explants were assessed for morphology and viability after 96 hours. In vivo study: AI-FeHAI were placed in 6 tarsocrural joints. Standard cytologic analysis and amikacin concentration (SFAC) were determined in synovia obtained regularly for 28 days thereafter. Similar analyses were conducted after a single intra-articular injection of amikacin 6 months later. In vitro study: Amikacin concentrations exceeded 16 μg/mL and inhibited S. aureus growth for 8 days. AI-FeHAI had no effect on cartilage explants. AI-FeHAI eliminated bacteria from synovial explants. In vitro study: After AI-FeHAI placement, SFAC was highest (140.78+63.81 μg/mL) at first sampling time. By 24 hours SFAC was