Thin-layer chromatography for the identification and semi-quantification of biogenic amines produced by bacteria

The screening TLC method described enables the simultaneous identification and semi-quantification of eight biogenic amines with a large number of samples handled in a short period of time. The dansylation process time was drastically reduced from 80 to 18 min by increasing the reaction temperature...

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Bibliographic Details
Published in:Journal of Chromatography A 2009-05, Vol.1216 (18), p.4128-4132
Main Authors: Latorre-Moratalla, M.L., Bover-Cid, S., Veciana-Nogués, T., Vidal-Carou, M.C.
Format: Article
Language:English
Subjects:
Amino acid decarboxylase activity
Bioconversions. Hemisynthesis
Biogenic amines
Biological and medical sciences
Biotechnology
Dansyl chloride
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Thin-layer chromatography
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Summary:The screening TLC method described enables the simultaneous identification and semi-quantification of eight biogenic amines with a large number of samples handled in a short period of time. The dansylation process time was drastically reduced from 80 to 18 min by increasing the reaction temperature to 100 °C. Bacteria could be classified as no, low, moderate or powerful amine producers when no spots were noticeable in TLC plates, less than 50 mg/L, from 50 to 500 mg/L, or more than 500 mg/L of amines were present in the decarboxylase broth medium, respectively. This TLC method is a simpler, faster and less expensive alternative to other methods, such as differential culture media, HPLC and even more sensitive than other TLC procedures.
ISSN:0021-9673
DOI:10.1016/j.chroma.2009.02.045