Effect of alkaline protease-producing Exiguobacterium sp. YS1 inoculation on the solubilization and bacterial community of waste activated sludge

A new approach to the solubilization of waste activated sludge (WAS) using an alkaline protease-producing bacterial isolate, Exiguobacterium sp. YS1, was investigated under controlled mild alkaline conditions at pH 10. Compared with the noninoculated experiment, the inoculated experiment in an anaer...

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Bibliographic Details
Published in:Bioresource technology 2009-10, Vol.100 (20), p.4597-4603
Main Authors: Lee, Sun Hee, Chung, Chung Wook, Yu, Young Joon, Rhee, Young Ha
Format: Article
Language:English
Subjects:
activated sludge
Alkaline protease
alkaline proteinases
anaerobic digestion
Anaerobiosis
Applied sciences
Bacillaceae - enzymology
Bacillaceae - genetics
Bacteria
Bacteria - isolation & purification
Bacterial Proteins - metabolism
Biological and medical sciences
Biological treatment of sewage sludges and wastes
Bioreactors - microbiology
Biotechnology
chemical oxygen demand
DGGE
Endopeptidases - metabolism
Environment and pollution
Exact sciences and technology
Exiguobacterium
Exiguobacterium sp. YS1
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Oxygen
Phylogeny
Pollution
proteinases
Sewage - microbiology
Solubility
Solubilization
Waste activated sludge
Waste Disposal, Fluid
Wastes
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Summary:A new approach to the solubilization of waste activated sludge (WAS) using an alkaline protease-producing bacterial isolate, Exiguobacterium sp. YS1, was investigated under controlled mild alkaline conditions at pH 10. Compared with the noninoculated experiment, the inoculated experiment in an anaerobic bioreactor increased soluble chemical oxygen demand concentration and alkaline protease activity by more than 40%, indicating a synergistic effect could be achieved when both bacterial inoculation and alkaline treatment were combined. Indeed, this combination led to 56.6% COD solubilization after 5 days of reaction time. However, the inoculant was not effective in the aerobic bioreactor. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA fragments revealed that the inoculated Exiguobacterium sp. YS1 became the predominant population in the bacterial community during the anaerobic solubilization processes. These results suggest that bioaugmentation of the organism might be useful for enhancing the solubilization of WAS at mild alkaline pH.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.04.056