Determination of aliphatic aldehydes in maize and grass silages using on-fibre derivatisation with O-(2,3,4,5,6-pentafluoro)benzylhydroxylamine

Aldehydes and ketones are volatile compounds of silages which probably affect feed preference, palatability and intake. A sensitive and fast method was developed to determine aliphatic aldehydes in maize and grass silages. The method is based on the solid phase micro-extraction and on-fibre derivati...

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Bibliographic Details
Published in:Animal feed science and technology 2009-06, Vol.152 (1), p.152-160
Main Authors: CHMELOVA, S, TRISKA, J, RUZICKOVA, K, KALAC, P
Format: Article
Language:English
Subjects:
Aldehydes
aliphatic aldehydes
Biological and medical sciences
chemical composition
corn silage
Determination
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
Grass silage
headspace analysis
Lactobacillus plantarum
Maize silage
O-(2,3,4,5,6-Pentafluoro)benzylhydroxylamine
On-fibre derivatisation
silage making
Solid phase micro-extraction
Zea mays
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Summary:Aldehydes and ketones are volatile compounds of silages which probably affect feed preference, palatability and intake. A sensitive and fast method was developed to determine aliphatic aldehydes in maize and grass silages. The method is based on the solid phase micro-extraction and on-fibre derivatisation of aldehydes with O-(2,3,4,5,6-pentafluoro)benzylhydroxylamine hydrochloride (PFBHA). The derivatisation agent was adsorbed onto divinylbenzene/poly(dimethylsiloxane) 65 μm fibre and then exposed to the aldehydes in the headspace of the silage samples. Aldehydes selectively react with PFBHA forming volatile oximes, which were desorbed in the injection port of a gas chromatograph and analysed by mass spectrometry. Using this method, eight aldehydes were detected in maize and grass silages being ethanal, propanal, butanal, 2-methylpropanal, pentanal, 3-methylbutanal, hexanal and heptanal, at levels ranging from tens to 200 mg/kg fresh matter. In two pilot laboratory experiments, contents of butanal and 3-methylbutanal decreased in silages prepared from grasses inoculated with Lactobacillus plantarum (1 × 10 5 CFU/g) or prepared from wilted forage.
ISSN:0377-8401
1873-2216
DOI:10.1016/j.anifeedsci.2009.03.012