Isolation and characterization of 17 polymorphic microsatellites in grass carp

Here we report the isolation and characterization of 17 polymorphic loci isolated from a partial genomic DNA library of grass carp (Ctenopharyngodon idellus) enriched for CA repeats. We tested variability of these microsatellites on 24 unrelated individuals collected in China. All microsatellites we...

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Bibliographic Details
Published in:Molecular ecology notes 2007-11, Vol.7 (6), p.1114-1116
Main Authors: LI, JIA LE, ZHU, ZE YUAN, WANG, GUI LING, BAI, ZHI YI, YUE, GEN HUA
Format: Article
Language:English
Subjects:
biological control
Ctenopharyngodon idella
Freshwater
grass carp
microsatellite repeats
microsatellites
polymorphism
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Summary:Here we report the isolation and characterization of 17 polymorphic loci isolated from a partial genomic DNA library of grass carp (Ctenopharyngodon idellus) enriched for CA repeats. We tested variability of these microsatellites on 24 unrelated individuals collected in China. All microsatellites were polymorphic. The average allele number was 7.9 per locus, ranging from four to 13. The observed heterozygosity was from 0.46 to 0.88 with an average of 0.71, whereas the average expected heterozygosity was 0.78. Sixteen of the 17 microsatellites conformed to Hardy-Weinberg equilibrium, and inherited independently. These microsatellites can be used to study genetic diversity and population structure of wild populations, and facilitate selective breeding of cultured broodstocks.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2007.01797.x