Light-up RNA aptamer enabled label-free protein detection via a proximity induced transcription assay

A novel proximity induced transcription assay for highly sensitive protein detection based on protein mediated ligation of a DNA template with the transcription of a light-up RNA aptamer for signal amplification has been developed.

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2018, Vol.54 (64), p.8877-8880
Main Authors: Ying, Zhan-Ming, Xiao, Hu-Yan, Tang, Hao, Yu, Ru-Qin, Jiang, Jian-Hui
Format: Article
Language:English
Subjects:
Antibodies - immunology
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Base Sequence
Biosensing Techniques - methods
Deoxyribonucleic acid
DNA
DNA - genetics
DNA - metabolism
DNA Ligases - metabolism
DNA Probes - genetics
DNA Probes - metabolism
DNA-Directed RNA Polymerases - metabolism
Fluorescent Dyes - chemistry
Fluoroimmunoassay - methods
Humans
Limit of Detection
Male
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Hybridization
Prostate-Specific Antigen - blood
Prostate-Specific Antigen - immunology
Proteins
Ribonucleic acid
RNA
Transcription, Genetic
Viral Proteins - metabolism
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Description
Summary:A novel proximity induced transcription assay for highly sensitive protein detection based on protein mediated ligation of a DNA template with the transcription of a light-up RNA aptamer for signal amplification has been developed.
ISSN:1359-7345
1364-548X
DOI:10.1039/c8cc04498h