Determination of Bisphenol-A in Water by Semi-Micro Column High-Performance Liquid Chromatography Using 2-Methoxy-4-(2-phthalimidinyl)-phenylsulfonyl Chloride as a Fluorescent Labeling Reagent

A highly sensitive method for the determination of bisphenol-A in water with semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent has been developed. The labeling reaction was carried out at 70°C for 20...

Full description

Saved in:
Bibliographic Details
Published in:Analytical Sciences 2005, Vol.21(6), pp.697-699
Main Authors: TSURUTA, Yasuto, INOUE, Hirofumi, FUKUNAGA, Keiko, MUNEMURA, Sayaka, OZAKI, Misa, OHTA, Masaya, MATSUURA, Fumito
Format: Article
Language:English
Subjects:
Benzhydryl Compounds
Chromatography, High Pressure Liquid
Estrogens, Non-Steroidal - analysis
Fluorescent Dyes
Fresh Water - analysis
Indicators and Reagents
Phenols - analysis
Phthalimides - chemistry
Reproducibility of Results
Spectrometry, Fluorescence
Sulfinic Acids - chemistry
Water Pollutants, Chemical - analysis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A highly sensitive method for the determination of bisphenol-A in water with semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent has been developed. The labeling reaction was carried out at 70°C for 20 min in borate buffer (pH 9.5). The derivative eluted at 11.6 min on a reversed-phase column with methanol-water (78:22, v/v) at a flow-rate of 0.2 ml/min. The fluorescence was monitored at 308 nm for excitation and 410 nm for emission. The detection limit (S/N = 3) was 10 fmol per injection. The labeling yield was about 95%.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.21.697