Two-Photon Fluorescent Turn-On Probe for Lipid Rafts in Live Cell and Tissue

We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1- dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts t...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2008-01, Vol.130 (13), p.4246-4247
Main Authors: Kim, Hwan Myung, Jeong, Byeong Ha, Hyon, Ju-Yong, An, Myoung Jin, Seo, Mun Sik, Hong, Jin Hee, Lee, Kyoung J, Kim, Chul Hoon, Joo, Taiha, Hong, Seok-Cheol, Cho, Bong Rae
Format: Article
Language:English
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Summary:We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1- dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mm in live tissues using two-photon microscopy.
ISSN:1272-7863
1520-5126
DOI:10.1021/ja711391fPII:S0002-7863(71)01391-X