In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout ( Oncorhynchus mykiss) erythrocytes

• Published in: Pesticide biochemistry and physiology 2009-10, Vol.95 (2), p.95-99
• Main Authors: Şentürk, Murat, Ceyhun, Saltuk Buğrahan, Erdoğan, Orhan, Küfrevioğlu, Ömer İrfan
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Brackish; Deltamethrin; enzyme activity; enzyme inhibition; Erythrocyte; erythrocytes; Freshwater; glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate dehydrogenase; Marine; Oncorhynchus mykiss; Pesticide; pesticides; trout
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2009.07.005

We investigated the in vitro and in vivo effects of some pesticides on rainbow trout erythrocyte glucose-6-phosphate dehydrogenase enzyme. The enzyme was purified 1691-fold with a specific activity of 16.235 U/mg protein and a yield of 63%. Cypermethrin, and propoxur inhibited glucose-6-phosphate dehydrogenase enzyme in vitro and deltamethrin inhibited both in vivo and in vitro. The obtained IC 50 values for deltamethrin, cypermethrin, and propoxur were 0.63, 1.02, and 12 mM, respectively. The activity of the control was determined as 5.17 ± 0.62 U/g Hb in in vivo studies. The enzyme activities of the groups treated with 0.25 g/L deltamethrin were measured at 6, 12, 24, 48, and 72 h, and found to be 4.32 ± 0.47, 3.57 ± 0.39, 3.47 ± 0.45, 2.86 ± 0.37, and 2.31 ± 0.32 U/g Hb. In vivo experiments showed that deltamethrin significantly inhibited the G6PD enzyme activity after the 48th h ( p < 0.05).