Genotoxic effects of white fluorescent light on human lymphocytes in vitro

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis 2008-04, Vol.652 (2), p.204-207
• Main Authors: Amorim, Marúcia Irena Medeiros, Ferrari, Íris, Bahia, Marcelo de Oliveira, Lima, Patrícia Danielle Lima, Cardoso, Plínio Cerqueira dos Santos, Khayat, André Salim, Cabral, Isabel Rosa, Burbano, Rommel Rodríguez
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chromosome aberrations; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Genotoxicity; Lymphocytes; Medical sciences; Mitotic index; SCE; Toxicology; White fluorescent light
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/j.mrgentox.2008.03.002

Sources of light beams such as white fluorescent light, are present in our daily life to meet the needs of life in the modern world. This study was conducted with the objective of determining the possible genotoxic, cytotoxic and aneugenic effects caused by this agent in different stages of the cell cycle (G0/early G1, S, and late G2), using different cytogenetic parameters (sister chromatid exchanges––SCE, chromosome aberrations––CA, and detection of aneugenic effects) in lymphocytes from temporary cultures of human peripheral blood. WFL showed a genotoxic effect in vitro, expressed by an increase in the frequency of SCE's, regardless of the cell cycle stage. However, no increase in the frequency of CAs was observed. In addition, disturbances in cell cycle kinetics and chromosomal segregation were also observed. Taken together, such data may contribute to a better understanding and a different management in the use of phototherapy for some pathological conditions.