FORMALIN-INDUCED INCREASE IN P2X3 RECEPTOR EXPRESSION IN DORSAL ROOT GANGLIA: IMPLICATIONS FOR NOCICEPTION

SUMMARY 1 ATP‐gated P2X receptors in nociceptive sensory neurons participate in the transmission of pain signals from the periphery to the spinal cord. The effect of formalin on the expression of P2X3 receptors in dorsal root ganglia (DRG) was characterized using molecular and immunological approach...

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Bibliographic Details
Published in:Clinical and experimental pharmacology & physiology 2009-08, Vol.36 (8), p.e6-e11
Main Authors: Pan, Ai-Hua, Lu, Da-Hua, Luo, Xue-Gang, Chen, Ling, Li, Zhi-Yuan
Format: Article
Language:English
Subjects:
dorsal root ganglia
P2X3 receptor
spinal cord
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Summary:SUMMARY 1 ATP‐gated P2X receptors in nociceptive sensory neurons participate in the transmission of pain signals from the periphery to the spinal cord. The effect of formalin on the expression of P2X3 receptors in dorsal root ganglia (DRG) was characterized using molecular and immunological approaches and the patch‐clamp technique. 2 Adult Sprague‐Dawley rats were injected with 100 µL of 5% formalin in the planar surface of the hindpaw and were killed 30 min and 1, 3, 6, 12, 24 and 48 h later for in vitro analyses. The expression and distribution of P2X3 receptors in the lumbar spinal cord and in L5/L6 DRG were examined; 24 and 48 h after formalin injection, currents in neurons were examined using whole‐cell patch‐clamp recording. 3 Western blots showed that anti‐P2X3 antibody recognized a major monomer of approximately 64 kDa in DRG. Immunoreactivity for P2X3 receptors was detected predominantly in the cytoplasm and plasma membrane of small (
ISSN:0305-1870
1440-1681
DOI:10.1111/j.1440-1681.2009.05179.x