Accumulation of tetrodotoxin and 4,9-anhydrotetrodotoxin in cultured juvenile kusafugu Fugu niphobles by dietary administration of natural toxic komonfugu Fugu poecilonotus liver

• Published in: Toxicon (Oxford) 2008-06, Vol.51 (7), p.1269-1273
• Main Authors: Kono, Michiko, Matsui, Takashi, Furukawa, Kiyoshi, Yotsu-Yamashita, Mari, Yamamori, Kunio
• Format: Article
• Language: English
• Subjects: 9-anhydroTTX; Administration, Oral; Animal Feed; Animal poisons toxicology. Antivenoms; Animals; Biological and medical sciences; Dietary administration; Food Contamination; Fugu; Fugu niphobles; Kusafugu Fugu niphobles; Life Cycle Stages - drug effects; Life Cycle Stages - physiology; Liver - chemistry; Liver - drug effects; Liver - metabolism; Medical sciences; Skin - chemistry; Skin - drug effects; Skin - metabolism; Sodium Channel Blockers - pharmacokinetics; Sodium Channel Blockers - toxicity; Takifugu - growth & development; Takifugu - metabolism; Tetrodotoxin; Tetrodotoxin - analogs & derivatives; Tetrodotoxin - pharmacokinetics; Tetrodotoxin - toxicity; Toxicology
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2008.02.017

Non-toxic cultured juvenile kusafugu Fugu niphobles were fed with a diet containing highly toxic natural komonfugu Fugu poecilonotus liver until the 30th day (8.0 μg of TTX and 3.7 μg of 4,9-anhydroTTX/fish/day), and then fed with a non-toxic diet until the 240th day. During the 30–240th day, five or six fish were periodically sampled six times, and the contents of TTX and 4,9-anhydroTTX in each tissue were determined. The total TTX and 4,9-anhydroTTX accumulated in all tissues tested was not significantly changed during the experimental period, both being kept at 70% of administrated doses. However, in the liver, the TTX content accounted to be 120 μg (50% of administrated) on the 30th day, and then it gradually decreased to 50 μg until the 240th day, while 4,9-anhydroTTX content was kept at approximately 40 μg (40% of administrated) during all the experimental periods. In contrast to the liver, in the skin, TTX and 4,9-anhydroTTX were 40 and 5 μg, respectively, on the 30th day, and then gradually increased to 80 and 24 μg, respectively, until the 240th day. In the intestine, TTX and 4,9-anhydroTTX contents were kept at 25 and 12 μg, respectively, during all the experimental periods. According to these results, we assumed that a part of TTX accumulated in the liver was slowly transferred to the skin.