pirA‐ and pirB‐like gene identification in Micrococcus luteus strains in Mexico

Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009 and afterwards in Mexico in 2013. AHPND is caused by Vibrio parahaemolyticus and affects Penaeus monodon and Litopenaeus vannamei shrimp cultures. The bacterium contains the pirA‐ and pirB‐like genes in 69‐ to 70‐Kb...

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Bibliographic Details
Published in:Journal of fish diseases 2018-11, Vol.41 (11), p.1667-1673
Main Authors: Durán‐Avelar, Ma. de Jesús, Vázquez‐Reyes, Alejandro, González‐Mercado, Ana Lourdes, Zambrano‐Zaragoza, José Francisco, Ayón‐Pérez, Miriam Fabiola, Agraz‐Cibrián, Juan Manuel, Gutiérrez‐Franco, Jorge, Vibanco‐Pérez, Norberto
Format: Article
Language:English
Subjects:
AHPND
Animals
Aquaculture
Bacteria
Bacterial Proteins - genetics
DNA
Farms
Gene sequencing
Genes
Genes, Bacterial - genetics
Hepatopancreas
Litopenaeus vannamei
Mexico
Microbiological strains
Micrococcus luteus
Micrococcus luteus - genetics
Necrosis
Nucleotide sequence
Outbreaks
PCR
Penaeidae - microbiology
Penaeus monodon
pirA‐ and pirB‐like genes
PirA‐ and PirB‐like toxin
Plasmids
Polymerase Chain Reaction - veterinary
Shellfish
Shellfish farming
Shrimp culture
Strains (organisms)
Toxins
Vibriosis
Waterborne diseases
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Summary:Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009 and afterwards in Mexico in 2013. AHPND is caused by Vibrio parahaemolyticus and affects Penaeus monodon and Litopenaeus vannamei shrimp cultures. The bacterium contains the pirA‐ and pirB‐like genes in 69‐ to 70‐Kb plasmids, which encode the toxins that produce the disease. The aim of this study was to determine whether pirA‐ and pirB‐like genes existed in bacterial genera distinct from Vibrio before the first cases of AHPND were documented in Mexico. Two bacterial isolates were selected from shrimp farms in Nayarit in 2006 and analysed by nested‐PCR to determine the presence of pirA‐ and pirB‐like genes. The two isolates chosen did indeed show the presence of these genes, and those findings were confirmed by sequencing. Both strains matched to the bacterial species Micrococcus luteus. Results revealed two important situations: (a) the pirA‐ and pirB‐like genes were present in a bacterial species that has not been reported previously (Micrococcus luteus); and (b) pirA‐ and pirB‐like bacterial genes were present in Mexico before the first AHPND outbreak was reported in China.
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.12874