Glucocorticoid Availability in Colonic Inflammation of Rat

• Published in: Digestive diseases and sciences 2008-08, Vol.53 (8), p.2160-2167
• Main Authors: Ergang, P., Leden, P., Bryndová, J., Žbánková, Š., Mikšík, I., Kment, M., Pácha, J.
• Format: Article
• Language: English
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism; 11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics; 11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism; Animals; Biochemistry; Biological and medical sciences; Budesonide - pharmacology; Carbenoxolone - pharmacology; Colitis - chemically induced; Colitis - metabolism; Colon - drug effects; Colon - enzymology; Colon - metabolism; Corticosterone - metabolism; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Disease Models, Animal; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology; Glucocorticoids - antagonists & inhibitors; Glucocorticoids - metabolism; Glucocorticoids - pharmacology; Hepatology; Hormone Antagonists - pharmacology; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Male; Medicine; Medicine & Public Health; Mifepristone - pharmacology; Mucin-2; Mucins - genetics; Mucins - metabolism; Oncology; Original Paper; Peroxidase - metabolism; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Transplant Surgery; Trinitrobenzenesulfonic Acid; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-007-0125-6

Recent in vitro studies have shown the involvement of pro-inflammatory cytokines in the regulation of the local metabolism of glucocorticoids via 11β-hydroxysteroid dehydrogenase type 1 and type 2 (11HSD1 and 11HSD2). However, direct in vivo evidence for a relationship among the local metabolism of glucocorticoids, inflammation and steroid enzymes is still lacking. We have therefore examined the changes in the local metabolism of glucocorticoids during colonic inflammation induced by TNBS and the consequences of corticosterone metabolism inhibition by carbenoxolone on 11HSD1, 11HSD2, cyclooxygenase 2 (COX-2), mucin 2 (MUC-2), tumor necrosis factor α (TNF-α), and interleukin 1β (IL-1β). The metabolism of glucocorticoids was measured in tissue slices in vitro and their 11HSD1, 11HSD2, COX-2, MUC-2, TNF-α, and IL-1β mRNA abundances by quantitative reverse transcription-polymerase chain reaction. Colitis produced an up-regulation of colonic 11HSD1 and down-regulation of 11HSD2 in a dose-dependent manner, and these changes resulted in a decreased capacity of the inflamed tissue to inactivate tissue corticosterone. Similarly, 11HSD1 transcript was increased in colonic intraepithelial lymphocytes of TNBS-treated rats. Topical intracolonic application of carbenoxolone stimulated 11HSD1 mRNA and partially inhibited 11HSD2 mRNA and tissue corticosterone inactivation and these changes were blocked by RU-486. The administration of budesonide mimicked the effect of carbenoxolone. In contrast to the local metabolism of glucocorticoids, carbenoxolone neither potentiates nor diminishes gene expression for COX-2, TNF-α, and IL-1β, despite the fact that budesonide down-regulated all of them. These data indicate that inflammation is associated with the down-regulation of tissue glucocorticoid catabolism. However, these changes in the local metabolism of glucocorticoids do not modulate the expression of COX-2, TNF-α, and IL-1β in inflamed tissue.