Elastin Stabilization Through Polyphenol and Ferric Chloride Combined Treatment for the Enhancement of Bioprosthetic Heart Valve Anticalcification

The lifetime of bioprosthetic heart valves (BHVs) is limited by the mechanical damage and calcification. The major components of BHVs are collagen and elastin. Collagen could be well protected by glutaraldehyde (GLUT) crosslinking, while elastin is not stabilized and has a high risk of degradation,...

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Bibliographic Details
Published in:Artificial organs 2018-11, Vol.42 (11), p.1062-1069
Main Authors: Jin, Wanyu, Guo, Gaoyang, Chen, Liang, Lei, Yang, Wang, Yunbing
Format: Article
Language:English
Subjects:
Animals
Anticalcification
Bioprosthesis
Bioprosthetic heart valves
Calcification
Calcinosis - prevention & control
Calcium
Calcium ions
Catechin - analogs & derivatives
Catechin - chemistry
Chlorides - chemistry
Collagen
Collagen - chemistry
Combined treatment
Cross-Linking Reagents - chemistry
Crosslinking
Cytotoxicity
Degradation
Elastase
Elastin
Elastin - chemistry
Epigallocatechin gallate
Ethanol
Ferric chloride
Ferric Compounds - chemistry
Glutaral - chemistry
Glutaraldehyde
Heart Valve Prosthesis
Heart valves
Iron
Iron chlorides
Male
Pericardium
Pericardium - chemistry
Pericardium - ultrastructure
Polyphenol
Polyphenols - chemistry
Protein Stability
Rats, Sprague-Dawley
Swine
Tannic acid
Tannins - chemistry
Tensile Strength
Time dependence
Toxicity testing
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Summary:The lifetime of bioprosthetic heart valves (BHVs) is limited by the mechanical damage and calcification. The major components of BHVs are collagen and elastin. Collagen could be well protected by glutaraldehyde (GLUT) crosslinking, while elastin is not stabilized and has a high risk of degradation, which could lead to the calcification of BHVs. We aimed to develop methods for stabilizing elastin and decreasing calcification. We investigated the combined tannic acid (TA) or epigallocatechin gallate (EGCG) with ferric chloride to stabilize elastin and prevent calcification. We found that the amount of TA/EGCG bound to elastin was in a time‐dependent pattern and this reaction showed better efficiency in acidic condition and ethanol‐water mixed solvents. Moreover, Fe3+ could compete with Ca2+ to bind to polyphenol, which could reduce the calcium deposition on BHVs. Cytotoxicity test showed that all extracts from different treatments had similar cell viabilities (85–100%). Through the combined treatments of polyphenol and ferric chloride, the pericardium had a better resistance to elastase degradation and more excellent anticalcification performance.
ISSN:0160-564X
1525-1594
DOI:10.1111/aor.13151