Improving Access to the Control Region and tRNA Gene Clusters of Dipteran Mitochondrial DNA

Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gen...

Full description

Saved in:
Bibliographic Details
Published in:Journal of medical entomology 2006-05, Vol.43 (3), p.636-639
Main Authors: Oliveira, M T, Da rosa, AC, Azeredo-espin, A, Lessinger, A C
Format: Article
Language:English
Subjects:
Calliphoridae
Muscidae
Oestridae
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gene clusters of dipterans of medical and veterinary importance, including Muscidae, Calliphoridae, and Oestridae species. These new primers were useful for investigating the nucleotide information and the structural organization of dipteran mtDNA.
ISSN:0022-2585
DOI:10.1603/0022-2585(2006)43[636:IATTCR]2.0.CO;2