Quantitative analysis of sugars in wood hydrolyzates with super(1)H NMR during the autohydrolysis of hardwoods

The focus of this work was to determine the utility of super(1)H NMR spectroscopy in the quantification of sugars resulting from the solubilization of hemicelluloses during the autohydrolysis of hardwoods and the use of this technique to evaluate the kinetics of this process over a range of temperat...

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Bibliographic Details
Published in:Bioresource technology 2009-12, Vol.100 (24), p.6398-6406
Main Authors: Mittal, Ashutosh, Scott, Gary M, Amidon, Thomas E, Kiemle, David J, Stipanovic, Arthur J
Format: Article
Language:English
Subjects:
Acer saccharum
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Summary:The focus of this work was to determine the utility of super(1)H NMR spectroscopy in the quantification of sugars resulting from the solubilization of hemicelluloses during the autohydrolysis of hardwoods and the use of this technique to evaluate the kinetics of this process over a range of temperatures and times. Yields of residual xylan, xylooligomers, xylose, glucose, and the degraded products of sugars, i.e., furfural and HMF (5-hydroxymethyl furfural), were determined. The monosaccharide and oligomer contents were quantified with a recently developed high resolution super(1)H NMR spectroscopic analysis. This method provided precise measurement of the residual xylan and cellulose remaining in the extracted wood samples and xylose and glucose in the hydrolyzates. NMR was found to exhibit good repeatability and provided carbohydrate compositional results comparable to published methods for sugar maple and aspen woods.
ISSN:0960-8524
DOI:10.1016/j.biortech.2009.06.107