Selection of Anti‐gluten DNA Aptamers in a Deep Eutectic Solvent

Herein, we show the feasibility of using deep eutectic solvents as a faster way of selecting aptamers targeting poorly water‐soluble species. This unexplored concept is illustrated for gluten proteins. In this way, aptamer‐based gluten detection can be performed directly in the extraction media with...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2018-09, Vol.57 (39), p.12850-12854
Main Authors: Svigelj, Rossella, Dossi, Nicolò, Toniolo, Rosanna, Miranda‐Castro, Rebeca, de‐los‐Santos‐Álvarez, Noemí, Lobo‐Castañón, M. Jesús
Format: Article
Language:English
Subjects:
Aptamers
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Base Sequence
Biotinylation
Deoxyribonucleic acid
DNA
Feasibility studies
Food safety
Gluten
Glutens - analysis
Glutens - metabolism
ionic liquids
Proteins
SELEX
SELEX Aptamer Technique - methods
Solvents
Solvents - chemistry
Water - chemistry
water-insoluble targets
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Herein, we show the feasibility of using deep eutectic solvents as a faster way of selecting aptamers targeting poorly water‐soluble species. This unexplored concept is illustrated for gluten proteins. In this way, aptamer‐based gluten detection can be performed directly in the extraction media with improved detectability. We envision deep implications for applications not only in food safety control but also in biomedicine. DES‐SELEX: Aptamers can be selected in deep eutectic solvents composed of choline chloride and 1,2‐ethanediol faster than in aqueous media. These highly solubilizing media, mimicking the intracellular environment, allow targeting non‐soluble or poorly water‐soluble species, which include targets of great interest in biomedical, pharmaceutical, and food analysis.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201804860