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Role of lymphocytes in the course of murine zymosan-induced peritonitis
. Objective and Design: To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis. Material or Subjects: Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group). Treatment: Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrifice...
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Published in: | Inflammation research 2008-06, Vol.57 (6), p.272-278 |
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creator | Kolaczkowska, E. Barteczko, M. Plytycz, B. Arnold, B. |
description | .
Objective and Design:
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.
Material or Subjects:
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).
Treatment:
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.
Methods:
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk’s staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE
2
, KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-
γ
, TNF-α, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student’s t-test (p ≤0.05).
Results:
During zymosan peritonitis, there was a decreased production of IFN-α (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.
Conclusions:
Lymphocytes do not have a significant impact on zymosan peritonitis in mice. |
doi_str_mv | 10.1007/s00011-007-7131-1 |
format | article |
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Objective and Design:
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.
Material or Subjects:
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).
Treatment:
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.
Methods:
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk’s staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE
2
, KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-
γ
, TNF-α, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student’s t-test (p ≤0.05).
Results:
During zymosan peritonitis, there was a decreased production of IFN-α (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.
Conclusions:
Lymphocytes do not have a significant impact on zymosan peritonitis in mice.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s00011-007-7131-1</identifier><identifier>PMID: 18516709</identifier><language>eng</language><publisher>Basel: SP Birkhäuser Verlag Basel</publisher><subject>Allergology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Capillary Permeability ; Chemokines - metabolism ; Cytokines - metabolism ; Dermatology ; Dinoprostone - metabolism ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Immunology ; Lymphocytes - immunology ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neurology ; Nitric Oxide - metabolism ; Peritonitis - chemically induced ; Peritonitis - immunology ; Pharmacology/Toxicology ; Protein Array Analysis ; Rheumatology ; Zymosan - pharmacology</subject><ispartof>Inflammation research, 2008-06, Vol.57 (6), p.272-278</ispartof><rights>Birkhaueser 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-98335b2fee9133dd221c4555a4cfd19cd53838da035ac9fa22fb89221e02467f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18516709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kolaczkowska, E.</creatorcontrib><creatorcontrib>Barteczko, M.</creatorcontrib><creatorcontrib>Plytycz, B.</creatorcontrib><creatorcontrib>Arnold, B.</creatorcontrib><title>Role of lymphocytes in the course of murine zymosan-induced peritonitis</title><title>Inflammation research</title><addtitle>Inflamm. res</addtitle><addtitle>Inflamm Res</addtitle><description>.
Objective and Design:
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.
Material or Subjects:
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).
Treatment:
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.
Methods:
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk’s staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE
2
, KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-
γ
, TNF-α, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student’s t-test (p ≤0.05).
Results:
During zymosan peritonitis, there was a decreased production of IFN-α (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.
Conclusions:
Lymphocytes do not have a significant impact on zymosan peritonitis in mice.</description><subject>Allergology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Capillary Permeability</subject><subject>Chemokines - metabolism</subject><subject>Cytokines - metabolism</subject><subject>Dermatology</subject><subject>Dinoprostone - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Immunology</subject><subject>Lymphocytes - immunology</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Neurology</subject><subject>Nitric Oxide - metabolism</subject><subject>Peritonitis - chemically induced</subject><subject>Peritonitis - immunology</subject><subject>Pharmacology/Toxicology</subject><subject>Protein Array Analysis</subject><subject>Rheumatology</subject><subject>Zymosan - pharmacology</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LxDAQxYMo7rr6AbxI8eAtmkmabXuURVdhQRAFbyGbP26XtqlJe6if3qxdWBA8zYP5zZuZh9AlkFsgJLsLhBAAHCXOgAGGIzSFlBJckPzjOGpCGWY5IxN0FsI20jnN6SmaQM5hnpFiipavrjKJs0k11O3GqaEzISmbpNuYRLneh99m3fuyMcn3ULsgG1w2uldGJ63xZeeasivDOTqxsgrmYl9n6P3x4W3xhFcvy-fF_QqrlJAOFzljfE2tMQUwpjWloFLOuUyV1VAozeO5uZaEcakKKym167yIlCE0nWeWzdDN6Nt699Wb0Im6DMpUlWyM64Og8UWesTSC13_AbXynibcJCnOapqzgEYIRUt6F4I0VrS9r6QcBROwiFmPEYid3EQuIM1d7435dG32Y2GcaAToCIbaaT-MPm_93_QHXm4W1</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Kolaczkowska, E.</creator><creator>Barteczko, M.</creator><creator>Plytycz, B.</creator><creator>Arnold, B.</creator><general>SP Birkhäuser Verlag Basel</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20080601</creationdate><title>Role of lymphocytes in the course of murine zymosan-induced peritonitis</title><author>Kolaczkowska, E. ; Barteczko, M. ; Plytycz, B. ; Arnold, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-98335b2fee9133dd221c4555a4cfd19cd53838da035ac9fa22fb89221e02467f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Allergology</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Capillary Permeability</topic><topic>Chemokines - metabolism</topic><topic>Cytokines - metabolism</topic><topic>Dermatology</topic><topic>Dinoprostone - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Immunology</topic><topic>Lymphocytes - immunology</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Neurology</topic><topic>Nitric Oxide - metabolism</topic><topic>Peritonitis - chemically induced</topic><topic>Peritonitis - immunology</topic><topic>Pharmacology/Toxicology</topic><topic>Protein Array Analysis</topic><topic>Rheumatology</topic><topic>Zymosan - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kolaczkowska, E.</creatorcontrib><creatorcontrib>Barteczko, M.</creatorcontrib><creatorcontrib>Plytycz, B.</creatorcontrib><creatorcontrib>Arnold, B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kolaczkowska, E.</au><au>Barteczko, M.</au><au>Plytycz, B.</au><au>Arnold, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of lymphocytes in the course of murine zymosan-induced peritonitis</atitle><jtitle>Inflammation research</jtitle><stitle>Inflamm. res</stitle><addtitle>Inflamm Res</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>57</volume><issue>6</issue><spage>272</spage><epage>278</epage><pages>272-278</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>.
Objective and Design:
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.
Material or Subjects:
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).
Treatment:
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.
Methods:
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk’s staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE
2
, KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-
γ
, TNF-α, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student’s t-test (p ≤0.05).
Results:
During zymosan peritonitis, there was a decreased production of IFN-α (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.
Conclusions:
Lymphocytes do not have a significant impact on zymosan peritonitis in mice.</abstract><cop>Basel</cop><pub>SP Birkhäuser Verlag Basel</pub><pmid>18516709</pmid><doi>10.1007/s00011-007-7131-1</doi><tpages>7</tpages></addata></record> |
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language | eng |
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source | Springer Nature |
subjects | Allergology Animals Biomedical and Life Sciences Biomedicine Capillary Permeability Chemokines - metabolism Cytokines - metabolism Dermatology Dinoprostone - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Immunology Lymphocytes - immunology Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - metabolism Mice Mice, Inbred C57BL Mice, Knockout Neurology Nitric Oxide - metabolism Peritonitis - chemically induced Peritonitis - immunology Pharmacology/Toxicology Protein Array Analysis Rheumatology Zymosan - pharmacology |
title | Role of lymphocytes in the course of murine zymosan-induced peritonitis |
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