Screening of β-fructofuranosidase-producing microorganisms and effect of pH and temperature on enzymatic rate

Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of β-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase acti...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2007-05, Vol.75 (1), p.87-93
Main Authors: Fernandez, Rubén Cuervo, Ottoni, Cristiane Angélica, da Silva, Elda Sabino, Matsubara, Rosa Mitiko Saito, Carter, José Márcio, Magossi, Luis Roberto, Wada, Maria Alice Alves, de Andrade Rodrigues, Maria Filomena, Maresma, Beatriz Guilarte, Maiorano, Alfredo Eduardo
Format: Article
Language:English
Subjects:
Aspergillus
Aspergillus niger
Aspergillus niger - enzymology
Aspergillus oryzae
Aspergillus oryzae - enzymology
beta-Fructofuranosidase - metabolism
Biological and medical sciences
Biotechnology
fructooligosaccharides
Fructosyltransferase
Fundamental and applied biological sciences. Psychology
Fungi - classification
Fungi - enzymology
Hexosyltransferases - metabolism
Hydrogen-Ion Concentration
Industrial Microbiology - methods
Oligosaccharides - metabolism
Saccharum - growth & development
Soil Microbiology
Temperature
β-fructofuranosidase
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Summary:Seventeen different strains of filamentous fungi were grown in batch cultures to compare their abilities for the production of β-fructofuranosidase. Three of them, Aspergillus oryzae IPT-301, Aspergillus niger ATCC 20611 and strain IPT-615, showed high production with total fructosyltransferase activity higher than 12,500 units l-¹. In addition, the β-fructofuranosidases of those strains have a high fructosyltransferase activity-to-hydrolytic activity ratio. The temperature and pH effects on the sucrose-β-fructofuranosidase reaction rate were studied using a 2² factorial experimental design. The comparative analysis of the tested variable coefficients shows that the variable pH contributes mostly to the changes in the fructosyltransferase and hydrolytic rates and in the V t/V h ratio. At 40 and 50°C, there were no significant differences between the fructosyltransferase and hydrolytic velocities of these enzymes.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-006-0803-x