Kinetics and spatial distribution of OTA in Aspergillus carbonarius cultures

The aim of this study was to characterize Ochratoxin A (OTA) production by Aspergillus carbonarius under different environmental conditions, and to elucidate the diffusion capacity of OTA throughout synthetic medium. One strain belonging to the species A. carbonarius isolated from vine dried fruit w...

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Bibliographic Details
Published in:Food microbiology 2006-12, Vol.23 (8), p.753-756
Main Authors: Valero, Ana, Farré, Joan R., Sanchis, Vicente, Ramos, Antonio J., Marín, Sonia
Format: Article
Language:English
Subjects:
A. carbonarius
Aspergillus - growth & development
Aspergillus - metabolism
Aspergillus carbonarius
Biological and medical sciences
biosynthesis
Colony Count, Microbial
Consumer Product Safety
Culture Media
food contamination
Food Contamination - analysis
Food Contamination - prevention & control
Food industries
Food Microbiology
food pathogens
Food toxicology
Fundamental and applied biological sciences. Psychology
inoculum density
Kinetics
molds (fungi)
nutrients
Ochratoxin A
Ochratoxins - biosynthesis
secondary metabolites
Synthetic nutrient medium
synthetic products
Temperature
Time Factors
toxic substances
toxigenic strains
Water - metabolism
water activity
water content
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Summary:The aim of this study was to characterize Ochratoxin A (OTA) production by Aspergillus carbonarius under different environmental conditions, and to elucidate the diffusion capacity of OTA throughout synthetic medium. One strain belonging to the species A. carbonarius isolated from vine dried fruit was single-point inoculated onto triplicate synthetic nutrient medium plates at two water activities (0.92 and 0.97) and two temperatures (20 and 30 °C). Daily radii were measured and OTA production was tested after 4, 7, 10, 14 and 18 days of incubation at four distances from the centre of colony (1–4 cm). OTA production was detected mainly at 0.97 a w. Earlier production was detected at 30 °C (optimum for growth), whereas maximum OTA concentrations were found at 20 °C. OTA production was detected from mycelium that was only a few days old and attained its optimum when mycelium was 4–7 days old at 0.97 a w. OTA diffusion was observed at 0.92 a w and 20 °C. Thus OTA production is discernable in young A. carbonarius mycelium and diffusion of the toxin has been shown to occur in a solid substrate.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2006.01.005