Evaluation of the immune responses following co-administration of PilQ and type b-flagellin from Pseudomonas aeruginosa in the burn mouse model

• Published in: Microbial pathogenesis 2018-10, Vol.123, p.426-432
• Main Authors: Bakht azad, Sima, Nikokar, Iraj, Faezi, Sobhan, Rasooly, Soheila, Mahdavi, Mehdi
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacterial Vaccines - immunology; Burn; Burns - immunology; Burns - microbiology; Cytokine; Cytokines - metabolism; Disease Models, Animal; Female; Fimbriae Proteins - administration & dosage; Fimbriae Proteins - genetics; Fimbriae Proteins - immunology; Flagellin; Flagellin - administration & dosage; Flagellin - genetics; Flagellin - immunology; Immunity, Humoral; Immunization; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunologic Factors - metabolism; Interferon-gamma - metabolism; Interleukin-4 - metabolism; Mice; Mice, Inbred BALB C; PilQ; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - immunology; Pseudomonas aeruginosa - pathogenicity; Pseudomonas Infections - immunology; Pseudomonas Infections - prevention & control; Recombinant Proteins; Spleen - immunology; Survival Rate; Wound Infection - immunology; Wound Infection - microbiology; Wound Infection - prevention & control
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2018.07.042

Considering the increased antibiotic resistance of Pseudomonas aeruginosa, the evaluation of immune response against the antigens of this bacterium seems necessary. In this study, the protective efficacy and immunological properties of P. aeruginosa recombinant PilQ (r-PilQ) and type b-flagellin (FLB) proteins was evaluated in the burn mouse model of infection. The inbred BALB/c mice were immunized with r-PilQ and FLB antigens. To investigate the type of induced immune response, sera were analyzed by ELISA for total IgG, IgG1, and IgG2a isotypes. After the final immunization, the IL-4, IFN-γ, and IL-17 cytokines level were examined in the spleen of non-challenged mice. Fifty days after lethal challenge, the survival rate and bacterial burden in the skin and other internal organs of experimental mice were assessed. The in vivo administration of r-PilQ, FLB and combined antigen resulted in a significant increase in the survival of mice (66%, 75%, and 83%, respectively) infected by the PAO1 strain of P. aeruginosa in the burn model of infection. Immunization of mice with r-PilQ and FLB mixture induced high titers of IL-4 and IL-17 cytokines compared to control groups (P