Investigation of the chemical profile and cytotoxicity evaluation of organic components eluted from pit and fissure sealants

• Published in: Food and chemical toxicology 2018-10, Vol.120, p.536-543
• Main Authors: Koulaouzidou, Elisabeth A., Roussou, Konstantina, Sidiropoulos, Konstantinos, Nikolaidis, Alexander, Kolokuris, Ioannis, Tsakalof, Andreas, Tsitsimpikou, Christina, Kouretas, Dimitrios
• Format: Article
• Language: English
• Subjects: Animals; Cytotoxicity; Dental sealants; Gas chromatography; Gas Chromatography-Mass Spectrometry; Mass sectrometry; Mice; Monomers; NIH 3T3 Cells; Organic Chemicals - chemistry; Organic Chemicals - toxicity; Pit and Fissure Sealants - chemistry; Pit and Fissure Sealants - toxicity; Polyethylene Glycols - chemistry; Polymethacrylic Acids - chemistry
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2018.07.042

The aim of this study was to identify organic components eluted from five resin dental sealants using gas chromatography and mass spectrometry (GC/MS) after 1-day and 40-days storage and the effect of sealants on cell survival of cultured fibroblasts. Five resin materials were studied: BeautiSealant (SHOFU), Clinpro (3M/ESPE), Conseal F (SDI), Grandio Seal (VOCO) and Helioseal Clear (Ivoclar/Vivadent). The organic monomers detected were butylated hydroxytoluene (BHT), bis-phenol-A (BPA), camphoroquinone (CQ), diethylenglycoldimethacrylate (DEGDMA), 4N, N-dimethylaminobenzoic acid butylethoxyester (DMABEE), hydroxyethylmethcrylate (HEMA), hydroquinone monomethylether (MEHQ), triethylene glycol dimethacrylate (TEGDMA), tetrabutylammonium tetrafluoroborate (TBATFB), triphenylstibane (TPSb). The main monomer detected was TEGDMA, whereas BHT and DEGDMA were detected at lower concentrations. Higher monomer concentrations were detected after 40 days storage. The eluting chemical profiles of the tested materials differ qualitative and quantitative. For cytotoxicity evaluation, NIH/3T3 cells were exposed to eluates of sealants and cell viability was assessed by a quantitative technique at two observation periods. Decreased cell viability was observed. The cytotoxicity and the release of monomers from dental materials examined depends on the type of material and the observation time point. Resin-based dental materials have raised public concerns regarding possible adverse biological effects, thus it is essential to evaluate possible side effects for human health. •Modern commercial dental sealants release small molecular weight monomers such as TEGDMA.•BPA was eluted from two materials after storage in methanol as detected by GC/MS.•A time-depended correlation seems to exist between cell viability and exposure to sealants.