Cloning and characterization of the promoter of the 9-cis-epoxycarotenoid dioxygenase gene in Arachis hypogaea L

We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. β-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by appl...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-09, Vol.73 (9), p.2103-2106
Main Authors: Liang, J.(South China Normal Univ., Guangzhou), Yang, L, Chen, X, Li, L, Guo, D, Li, H, Zhang, B
Format: Article
Language:English
Subjects:
9-cis-epoxycarotenoid dioxygenase
ABA
abscisic acid response element
ACIDE SALICYLIQUE
ACIDO SALICILICO
Arabidopsis
Arachis - enzymology
ARACHIS HYPOGAEA
Arachis hypogaea L
Base Sequence
Biological and medical sciences
Cloning, Molecular
Dioxygenases
DNA Primers
DNA, Plant
DROUGHT STRESS
ESTRES DE SEQUIA
ESTRES OSMOTICO
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Glucuronidase - genetics
Molecular Sequence Data
OSMOTIC STRESS
Oxygenases - genetics
Plant Proteins
Polymerase Chain Reaction
promoter
Promoter Regions, Genetic
SALICYLIC ACIDS
STRESS DU A LA SECHERESSE
STRESS OSMOTIQUE
water stress
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Summary:We cloned the promoter of the 9-cis-epoxycarotenoid dioxygenase gene from Arachis hypogaea L. β-Glucuronidase (GUS) histochemical staining and GUS activity assay indicated that the activity of the promoter was exhibited predominantly in the leaves and enhanced by water and NaCl stresses, and by application of abscisic acid (ABA) and salicylic acid (SA) in transgenic Arabidopsis. Moreover, two novel ABRE-like (abscisic acid response element) elements were identified in the promoter region.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.90133