Study of a room temperature phosphorescence phenomenon to allow the detection of aflatoxigenic strains in culture media

A novel screening method based on room temperature phosphorescence (RTP) for the visual detection of aflatoxigenic strains from Aspergillus genus is described. Strains were cultured on media widely used in food mycology to which methyl-β-cyclodextrin plus bile salts (0.6% sodium deoxycholate) were a...

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Bibliographic Details
Published in:International journal of food microbiology 2007-04, Vol.115 (2), p.149-158
Main Authors: Rojas-Durán, T.R., Fente, C.A., Vázquez, B.I., Franco, C.M., Sanz-Medel, A., Cepeda, A.
Format: Article
Language:English
Subjects:
aflatoxin B1
Aflatoxin production
aflatoxins
Aflatoxins - biosynthesis
Aflatoxins - isolation & purification
ambient temperature
Aspergillus
Aspergillus - classification
Aspergillus - growth & development
Aspergillus - isolation & purification
Aspergillus - metabolism
Aspergillus sp
Bile salts
Biological and medical sciences
Colony Count, Microbial
culture media
Culture Media - chemistry
Cyclodextrin
cyclodextrins
Cyclodextrins - metabolism
Fluorescence
food contamination
Food industries
Food microbiology
food pathogens
Fundamental and applied biological sciences. Psychology
General aspects
in vitro culture
Methods of analysis, processing and quality control, regulation, standards
microbial detection
Mycological Typing Techniques - methods
phosphorescence
Room temperature phosphorescence
serotypes
Species Specificity
Temperature
Time Factors
toxigenic strains
ultraviolet radiation
Ultraviolet Rays
UV light
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Description
Summary:A novel screening method based on room temperature phosphorescence (RTP) for the visual detection of aflatoxigenic strains from Aspergillus genus is described. Strains were cultured on media widely used in food mycology to which methyl-β-cyclodextrin plus bile salts (0.6% sodium deoxycholate) were added. Aflatoxin production was readily detectable after 3 days of incubation at 28 °C by RTP emission from the mycelium of aflatoxigenic strains observed after exposure to UV light. The method was tested on thirty-two Aspergillus sp. strains. The phosphorescence phenomenon was reproduced in vitro by immobilizing aflatoxin B 1 on ion exchange resin beads.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2006.10.018