Comprehensive study of endocrine disrupting compounds using grab and passive sampling at selected wastewater treatment plants in South East Queensland, Australia

• Published in: Environment international 2007-07, Vol.33 (5), p.654-669
• Main Authors: Tan, Benjamin L.L., Hawker, Darryl W., Müller, Jochen F., Leusch, Frédéric D.L., Tremblay, Louis A., Chapman, Heather F.
• Format: Article
• Language: English
• Subjects: Activated sludge; Applied sciences; Biological and medical sciences; Biological treatment of waters; Bioreactors; Biotechnology; Cell Line, Tumor; Cell Proliferation - drug effects; E-Screen; Endocrine disruptors; Endocrine Disruptors - analysis; Endocrine Disruptors - toxicity; Environment and pollution; Environmental Monitoring - instrumentation; Environmental Monitoring - methods; Estrogens - analysis; Estrogens - toxicity; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Gas chromatography–mass spectrometry; Humans; Industrial applications and implications. Economical aspects; Other wastewaters; Pollution; Queensland; Sewage - analysis; Solid-phase extraction; Stir bar sorptive extraction; Time-integrated sampler; Waste Disposal, Fluid; Wastewater treatment plant; Wastewaters; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity; Water treatment and pollution
• ISSN: 0160-4120; 1873-6750
• DOI: 10.1016/j.envint.2007.01.008

Chemical (gas chromatography–mass spectrometry, GC–MS) and biological (E-Screen assay) analyses were used to determine the concentrations of 15 endocrine disrupting compounds (EDCs) and estrogen equivalent (EEq) in grab and passive samples from five municipal wastewater treatment plants (WWTPs) in South East Queensland, Australia. EEq concentrations derived by E-Screen assays for the grab samples were between 108–356 ng/L for the influents and < 1–14.8 ng/L for the effluents with the exception of one effluent sample which was at 67.8 ng/L EEq. The EDC concentrations and EEq values for the passive samples were several times lower than those of the grab samples: a decrease probably caused by, but not limited to biofouling, low flow rate, biodegradation and temperature which can progressively reduce the uptake of compounds into the sampler. At this stage, grab sampling is the most reliable method for field monitoring; nevertheless, passive sampler is a useful sampling tool but the method requires more research to ensure that the information obtained can be interpreted appropriately. Although alkylphenols and phthalates were detected at higher concentrations in the wastewater samples as compared to natural hormones, the environmental risk may be negligible as their estrogenic potencies are several orders of magnitude lower than that of the natural estrogens. In most wastewater samples, the natural estrogens contributed to 60% or more of the EEq value. Removal efficacy of most estrogenic and xenoestrogenic compounds from the conventional activated sludge or biological nutrient removal (BNR) WWTPs monitored in this study was in the range of 80–> 99%. The efficiency of the WWTPs in removing estrogenic activity was > 95%. The EEqs of the E-Screen and those calculated from the results of extensive chemical analyses using the estradiol equivalency factors were comparable for most of the WWTPs samples.