Isolation, purification, and enzymatic activity of cellulase components of the fungus Aspergillus terreus

The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cell...

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Bibliographic Details
Published in:Chemistry of natural compounds 2007-09, Vol.43 (5), p.594-597
Main Authors: Mirzaakhmedov, Sh. Ya, Ziyavitdinov, Zh. F., Akhmedova, Z. R., Saliev, A. B., Ruzmetova, D. T., Ashurov, Kh. B., Fessas, Dimitrios, Iametti, Stefania
Format: Article
Language:English
Subjects:
Aspergillus terreus
Basidiomycetes
Micromycetes
Pleurotus ostreatus
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Summary:The accumulation dynamics of cellulolytic enzymes in culture media of the basidiomycete fungi Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during a long incubation period. It was found that A. terreus was the most active producer of cellulolytic enzymes among the studied fungi. Two protein fractions with cellulase activity were isolated using gel filtration and ion-exchange chromatography. PAAG electrophoresis showed that fraction-I consisted of four components; fraction-II, an electrophoretically homogeneous protein.
ISSN:0009-3130
1573-8388
DOI:10.1007/s10600-007-0199-6