Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics

Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughp...

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Bibliographic Details
Published in:Lab on a chip 2018-01, Vol.18 (18), p.2826-2837
Main Authors: Kwon, Taehong, Yao, Rujie, Hamel, Jean-François P, Han, Jongyoon
Format: Article
Language:English
Subjects:
Acoustophoresis
Animals
Bioreactors
Biotechnology
Cell Separation - instrumentation
Cell Survival
CHO Cells
Cricetulus
Dielectrophoresis
Equipment Design
Lab-On-A-Chip Devices
Lateral displacement
Microfluidics
Suspensions
Time Factors
Viability
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Summary:Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic cell separation devices based on dielectrophoresis, acoustophoresis, and deterministic lateral displacement are used to remove nonviable cells. However, their volumetric throughputs and test cell concentrations are generally too low to be useful in typical bioreactors in biomanufacturing. In this study, we demonstrate the efficient removal of small (
ISSN:1473-0197
1473-0189
DOI:10.1039/c8lc00250a