Sequence of plasmid pBS228 and reconstruction of the IncP-1α phylogeny

The antibiotic resistance plasmid pBS228 has been completely sequenced, and revealed to be descended from a plasmid virtually identical to the Birmingham IncP-1α plasmid RK2/RP4/RP1. However, it has three additional transposon insertions, one of which is responsible for the extra antibiotic resistan...

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Bibliographic Details
Published in:Plasmid 2007-07, Vol.58 (1), p.76-83
Main Authors: Haines, Anthony S., Jones, Karen, Batt, Sarah M., Kosheleva, Irina A., Thomas, Christopher M.
Format: Article
Language:English
Subjects:
Ampicillin resistance
Antibiotic resistance
Broad host range
Evolution
Tn1
Transposable element
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Summary:The antibiotic resistance plasmid pBS228 has been completely sequenced, and revealed to be descended from a plasmid virtually identical to the Birmingham IncP-1α plasmid RK2/RP4/RP1. However, it has three additional transposon insertions, one of which is responsible for the extra antibiotic resistances conferred. Loss of kanamycin resistance, which is characteristic of most IncP-1α plasmids, is the result of this insertion. A second transposon causes inactivation of the mating pair formation apparatus, rendering the plasmid non-self-transmissible. Comparison with the published data for other IncP-1α plasmids gives insight into the recent evolutionary history of this group as well as the acquisition and transmission of one of the first ampicillin resistance transposons discovered.
ISSN:0147-619X
1095-9890
DOI:10.1016/j.plasmid.2007.01.001