Molecular cloning and functional analysis of a UV-B photoreceptor gene, BpUVR8 (UV Resistance Locus 8), from birch and its role in ABA response

• Published in: Plant science (Limerick) 2018-09, Vol.274, p.294-308
• Main Authors: Li, Xiaoyi, Ma, Minghao, Shao, Wanxuan, Wang, Hengtao, Fan, Ruixin, Chen, Xiaohui, Wang, Xigang, Zhan, Yaguang, Zeng, Fansuo
• Format: Article
• Language: English
• Subjects: ABA; Betula platyphylla; Molecular cloning; UVR8
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2018.06.006

•The expression pattern of BpUVR8 was analyzed.•The function of UVR8 is conserved between Arabidopsis and Betula platyphylla.•BpUVR8 is a positive regulator in UV-B-induced photomorphogenesis in plants.•BpUVR8 functions as a negative regulator of ABA responses in the initial stage of germination and growth. As a photoreceptor specifically for UV-B light, UVR8 gene plays an important role in the photomorphogenesis and developmental growth of plants. In this research, we isolated the UVR8 gene from birch, named BpUVR8 (AHY02156). BpUVR8 overexpression rescued the uvr8 mutant phenotype using functional complementation assay of BpUVR8 in Arabidopsis uvr8 mutants, which showed that the function of UVR8 is conserved between Arabidopsis and birch. The expression analysis of BpUVR8 indicated that this gene is expressed in various tissues, but its expression levels in leaves are higher than in other organs. Moreover, abiotic stress factors, such as UV-B, salinity, and abscisic acid (ABA) can induce the expression of BpUVR8 gene. Interestingly, the analysis of promoter activity indicated that BpUVR8 promoter not only has the promoting activity but can also respond to the induction of abiotic stress and ABA signal. So, we analyzed its function in ABA response via transgenic UVR8 overexpression in Arabidopsis. The BpUVR8 enhances the susceptibility to ABA, which indicates that BpUVR8 is regulated by ABA and can inhibit seed germination. The root length of 20-day-old 35S::BpUVR8/WT transgenic plants was 18% reduced as compared to the wild-type under the ABA treatment. The membrane of the BpUVR8-overexpressing in Arabidopsis thaliana was the most damaged after ABA treatment and 35S::BpUVR8/WT transgenic plant was more sensitive to ABA than the wild type. These results showed that BpUVR8 is a positive regulator in the ABA signal transduction pathway. In the presence of low dose of UV-B, the sensitivity of wild-type and 35S::BpUVR8/WT plants to ABA was reduced. Moreover, BpUVR8 regulates the expression of a subset of ABA-responsive genes, both in Arabidopsis and Betula platyphylla, under the ABA treatment. Our data provide evidence that BpUVR8 is a positive regulator in the UV-B-induced photomorphogenesis in plants. Moreover, we propose from this research that BpUVR8 might have an important role in integrating plant growth and ABA signaling pathway.