Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens

• Published in: Veterinary microbiology 2004-01, Vol.98 (1), p.37-43
• Main Authors: Methner, U, Barrow, P.A, Gregorova, D, Rychlik, I
• Format: Article
• Language: English
• Subjects: Animals; Applied microbiology; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Cecum - microbiology; Chickens; Colonisation-inhibition; Colony Count, Microbial - veterinary; Fundamental and applied biological sciences. Psychology; Live vaccine; Liver - microbiology; Microbiology; Miscellaneous; Mutagenesis, Insertional; phoP mutants; Porins - genetics; Porins - immunology; Poultry Diseases - immunology; Poultry Diseases - microbiology; Poultry Diseases - prevention & control; Salmonella; Salmonella enteritidis - genetics; Salmonella enteritidis - growth & development; Salmonella enteritidis - immunology; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - prevention & control; Salmonella typhimurium; Salmonella typhimurium - genetics; Salmonella typhimurium - growth & development; Salmonella typhimurium - immunology; Salmonella Vaccines - genetics; Salmonella Vaccines - immunology; Sigma Factor - genetics; Sigma Factor - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Virulence
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2003.10.019

Administration of live Salmonella strains to day-old chicks provides profound protection against superinfection with a related strain within a matter of hours by a colonisation-inhibition mechanism, which is primarily a bacterial physiological process. Although currently available, commercial, live attenuated Salmonella vaccines induce protection by adaptive immunity, none of them is able to induce protection against Salmonella organisms by colonisation-inhibition and, therefore, they are unable to protect newly-hatched birds immediately after oral vaccination. In this study, mutants of Salmonella Typhimurium and Enteritidis with deletions in phoP and rpoS, either alone or in combination with ompC, were characterised and tested for their level of attenuation and their ability to inhibit the intestinal colonisation of the isogenic parent strains in chickens. Mutants with deletions only in rpoS demonstrated an unaffected potential to inhibit the intestinal colonisation of the challenge strain but were still fully virulent for the chickens. Mutants with deletions in phoP, either alone or in combination with rpoS, resulted in a high level of attenuation, unimpaired ability to colonise the gut and a nearly unaffected potential to inhibit the challenge strain from caecal colonisation. Mutants with an additional deletion in ompC revealed a reduced capacity of intestinal colonisation-inhibition when compared to the control strains and both the single rpoS and the phoP deletion mutants. Mutations in phoP- or phoP-regulated genes may therefore be used for the development of live attenuated Salmonella vaccines possessing these novel characteristics.