Regulation of synthesis of the pectolytic enzymes of Aspergillus niger

The synthesis of pectolytic enzymes appears to be considerably influenced by the glucose content of the cultivation medium containing mixed carbon sources in a predetermined optimal ratio. Glucose is an essential component in the fermentation, promoting cell growth while the synthesis of pectolytic...

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Bibliographic Details
Published in:Enzyme and microbial technology 2004-04, Vol.34 (5), p.466-473
Main Authors: Panda, T., Nair, Sushma R., Kumar, M.Prem
Format: Article
Language:English
Subjects:
Aspergillus niger
Biological and medical sciences
Biotechnology
Competitive inhibition
Corn
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Glucose
Induction
Methods. Procedures. Technologies
Pectolytic enzymes
Production of selected enzymes
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Summary:The synthesis of pectolytic enzymes appears to be considerably influenced by the glucose content of the cultivation medium containing mixed carbon sources in a predetermined optimal ratio. Glucose is an essential component in the fermentation, promoting cell growth while the synthesis of pectolytic enzymes was found to be induced by the second carbon source, corn, only when the glucose concentration in the medium was significantly low. Studies conducted to examine the effect of glucose showed that glucose severely reduced the synthesis of polygalacturonase and polymethylgalacturonase at concentrations above 5 g/l while pectinlyase was unaffected. In vitro studies showed that the inhibition of the two pectin hydrolases was reversible and competitive in nature. The results of the study also indicate induction of pectolytic enzymes by corn in Aspergillus niger NCIM 548.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2003.12.004