Construction of new stable strain over-expressing the glucose isomerase of the Streptomyces sp. SK strain

In order to over express the xylA gene of Streptomyces sp. SK strain, it was cloned under the control of the constitutive ermE-up promoter. This construct was integrated through site-specific recombination process into the chromosome of a Streptomyces violaceoniger glucose isomerase deficient strain...

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Bibliographic Details
Published in:Enzyme and microbial technology 2005-12, Vol.37 (7), p.735-738
Main Authors: Mezghani, Monia, Borgi, Mohamed Ali, Kammoun, Radhouane, Aouissaoui, Hedi, Bejar, Samir
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
CBS4 strain
ermE- up promoter
Fundamental and applied biological sciences. Psychology
Glucose isomerase
Over-expression
Streptomyces
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Summary:In order to over express the xylA gene of Streptomyces sp. SK strain, it was cloned under the control of the constitutive ermE-up promoter. This construct was integrated through site-specific recombination process into the chromosome of a Streptomyces violaceoniger glucose isomerase deficient strain using the non-replicative vector pTS55. The resulting CBS4 strain shows a perfect stability in the absence of selection pressure. Its glucose isomerase activity was about four and nine-fold greater, than that obtained from Streptomyces sp. SK, respectively fully induced or not by xylose.
ISSN:0141-0229
1879-0909
DOI:10.1016/j.enzmictec.2005.04.012