Coupling occurs before breakdown during biotransformation of Acid Blue 62 by white rot fungi

• Published in: Chemosphere (Oxford) 2008, Vol.70 (6), p.1097-1107
• Main Authors: Vanhulle, Sophie, Enaud, Estelle, Trovaslet, Marie, Billottet, Ludovic, Kneipe, Laurence, Jiwan, Jean-Louis Habib, Corbisier, Anne-Marie, Marchand-Brynaert, Jacqueline
• Format: Article
• Language: English
• Subjects: Acid Blue 62; Acids; anthraquinones; Anthraquinonic dye; Applied sciences; biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biological treatment of waters; Bioremediation; Biotechnology; biotransformation; Capillary electrophoresis; chemical reactions; Chromatography, High Pressure Liquid; Coloring Agents - chemistry; Coloring Agents - metabolism; Coriolopsis polyzona; Coriolus versicolor; dyes; Electrophoresis, Capillary; Environment and pollution; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Fungi - metabolism; Industrial applications and implications. Economical aspects; Industrial wastewaters; Magnetic Resonance Spectroscopy; Mass spectrometry; Metabolic pathway; Molecular Structure; Perenniporia; Perenniporia ochroleuca; Perenniporia tephropora; Pollution; Pycnoporus sanguineus; Spectrometry, Mass, Electrospray Ionization; Wastewaters; Water treatment and pollution; white-rot fungi
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2007.07.069

Only few data exist on the metabolites produced during the biotransformation of anthraquinonic dyes by white rot fungi (WRF). During the biotransformation of an anthraquinonic dye Acid Blue 62 (ABu62) using Pycnoporus sanguineus MUCL 41582 strain, it was previously demonstrated that the blue colour of the medium turned to red before complete dye decolourisation. To better understand the phenomenon, this study carried out ABu62 biotransformation with five different WRF strains ( Coriolopsis polyzona MUCL 38443, Perenniporia ochroleuca MUCL 41114, Perenniporia tephropora MUCL 41562, P. sanguineus MUCL 38531 and Trametes versicolor MUCL 38412) and compared with P. sanguineus MUCL 41582 previously described. A multi-methodological approach (using capillary electrophoresis, mass spectrometry, HPLC, UV, NMR and IR spectroscopies) was developed to characterise the metabolites involved and monitor their apparition. Seven metabolites were commonly found with all strains, suggesting a common metabolic pathway for ABu62 biotransformation. During the first days, dimer and oligomers of the initial ABu62 molecule were observed: the main one absorbed in the 500 nm region, explaining the red colour appearance of the medium. This main metabolite was made up of two molecules of ABu62 linked by an azo bond, minus a cyclohexyl moiety. After a longer incubation time, breakdown products were observed. Based on these products characterizations, a bioconversion pathway was proposed.