Study of genetic variation of different Cauliflower mosaic virus isolates infecting canola plant in Iran

Cauliflower mosaic virus (CaMV) is a type member of the genus Caulimovirus, from Caulimoviridae family. This virus is one of the most destructive viral diseases. The goal of this study was to detect the CaMV from canola plants. To do so, a number of 560 leaf samples showing various virus-like sympto...

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Bibliographic Details
Published in:Phytopathology 2009-06, Vol.99 (6), p.S43-S43
Main Authors: Ghaderi, M, Shahraeen, N, Rakhshandehroo, F
Format: Article
Language:English
Subjects:
Cauliflower mosaic virus
Caulimovirus
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Summary:Cauliflower mosaic virus (CaMV) is a type member of the genus Caulimovirus, from Caulimoviridae family. This virus is one of the most destructive viral diseases. The goal of this study was to detect the CaMV from canola plants. To do so, a number of 560 leaf samples showing various virus-like symptoms was collected from Varamin (Tehran province), Ghazvin and Takestan (Ghazvin province), Shahrekord (Chaharmahal-e-Bakhtiari province), Sari (Mazandaran province), Moghan (Ardabil province), Naghadeh (Azarbayejan-e-gharbi province) and Shiraz (Fars province). Using DAS-ELISA method, samples were tested by polyclonal antisera (DSMZ. Inc.) for the presence of CaMV. According to the results obtained by DAS-ELISA method, it is revealed that the above mentioned provinces are infected with the CaMV 38.4%, 36.8%, 43.3%, 31.8%, 66.6%, 52.3% and 38.2%, respectively. Using PCR molecular method and specific primers designed for the initial sequence of the ORF V of the viral genome sequence, presence of the CaMV was confirmed for the ELISA positive-tested canola plants. All infected tested samples amplified a 840-bp fragment in PCR reaction. The amplified fragments of four isolates have first been sequenced and then aligned with the corresponding data available for other CaMV isolates in NCBI. Phylogenetic analysis revealed that all these Iranian isolates together with two NCBI isolates (Cabb-D/H, Xingjiang) were categorized in one cluster while all other CaMV isolates from NCBI were categorized in a separate cluster.
ISSN:0031-949X