Enhanced biosynthesis of phenazine-1-carboxamide by Pseudomonas chlororaphis strains using statistical experimental designs

• Published in: World journal of microbiology & biotechnology 2018-09, Vol.34 (9), p.129-10, Article 129
• Main Authors: Peng, Huasong, Tan, Jian, Bilal, Muhammad, Wang, Wei, Hu, Hongbo, Zhang, Xuehong
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Biochemistry; Biological control; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Design of experiments; Environmental Engineering/Biotechnology; Experimental design; Genetic modification; Glycerol; Life Sciences; Microbiology; Original Paper; Peptones; Pesticides; Phenazine; Plant growth; Pseudomonas; Pseudomonas chlororaphis; Response surface methodology; Rhizosphere; Statistics
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-018-2501-0

Phenazine-1-carboxamide (PCN) is one of the major biocontrol agents produced by plant growth-promoting rhizosphere (PGPR) pseudomonads including Pseudomonas chlororaphis . In this study, a combined strategy of genetic modification and statistical experimental designs was applied to obtain mutants of P. chlororaphis strains with high-yield PCN production. To achieve this, the lon gene was knocked out in wild-type P. chlororaphis HT66 and the breeding mutant P3 strain with a non-scar deletion strategy. The resulting HT66Δ lon and P3Δ lon mutants produced a significantly higher PCN production in shake-flask cultures which was 5- and  9-folds greater than their native counterparts. The potential ability of strain P3 Δlon for PCN production was further optimized by statistical designs. A two-level Plackett–Burman (PB) experimental design with six variables was employed to scrutinize medium components that significantly influence PCN production. Notably, glycerol, tryptone, and soy peptone were identified to be the most significant factors ( p