Mapping a gene on wheat chromosome 4BL involved in a complementary interaction with adult plant leaf rust resistance gene LrSV2

• Published in: Theoretical and applied genetics 2018-11, Vol.131 (11), p.2333-2344
• Main Authors: Diéguez, María José, Petignat, Camila, Ferella, Luciana, Fiorentino, Gabriela, Silva, Martha, Dabove, Marisol Alicia, Rosero Yañez, Gustavo Iván, López, Micaela, Pergolesi, María Fernanda, Ingala, Lorena, Cuyeu, Alba Romina, Sacco, Francisco
• Format: Article
• Language: English
• Subjects: Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome mapping; Chromosomes; Gene mapping; Genes; Genetic aspects; Inbreeding; Leaf rust; Leaves; Life Sciences; Microsatellites; Microsatellites (Genetics); Original Article; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant resistance; Puccinia triticina; Seedlings; Triticum aestivum; Wheat
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-018-3155-x

Key message A complementary gene to LrSV2 for specific adult plant leaf rust resistance in wheat was mapped on chromosome 4BL, tightly linked to Lr12 / 31. LrSV2 is a race-specific adult plant leaf rust ( Puccinia triticina ) resistance gene on subdistal chromosome 3BS detected in the cross of the traditional Argentinean wheat ( Triticum aestivum ) variety Sinvalocho MA and the experimental line Gama6. The analysis of the cross of R46 [recombinant inbred line (RIL) derived from Sinvalocho MA carrying LrSV2 gene and the complementary gene Lrc - SV2 identified in the current paper] and the commercial variety Relmo Siriri (not carrying neither of these two genes) allowed the detection of the unlinked complementary gene Lrc - SV2 because the presence of one dominant allele of both is necessary to express the LrSV2 -specific adult plant resistance. Lrc - SV2 was mapped within a 1-cM interval on chromosome 4BL using 100 RILs from the cross Sinvalocho MA × Purple Straw. This genetic system resembles the Lr27+31 seedling resistance reported in the Australian varieties Gatcher and Timgalen where interacting genes map at similar chromosomal positions. However, in high-resolution maps, Lr27 and LrSV2 were already mapped to adjacent intervals on 3BS and Lrc - SV2 map position on 4BL is distal to the reported Lr12 / 31 -flanking microsatellites.