Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme

•Three polysaccharides were obtained under different culture conditions.•Glu-CPS-1 exhibited the highest antioxidant activity in vitro.•Proper culture conditions can enhance the antioxidant activity of polysaccharides. Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and pur...

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Bibliographic Details
Published in:Carbohydrate polymers 2018-10, Vol.198, p.426-433
Main Authors: Shen, Shi-gang, Jia, Shi-ru, Wu, Yi-kai, Yan, Rong-rong, Lin, Ya-Hui, Zhao, Dong-xue, Han, Pei-pei
Format: Article
Language:English
Subjects:
Antioxidant activity
Antioxidants - chemistry
Antioxidants - metabolism
Bacteriological Techniques
Culture condition
Nostoc - metabolism
Nostoc flagelliforme polysaccharides
Physicochemical property
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - metabolism
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Summary:•Three polysaccharides were obtained under different culture conditions.•Glu-CPS-1 exhibited the highest antioxidant activity in vitro.•Proper culture conditions can enhance the antioxidant activity of polysaccharides. Three polysaccharides (WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1) were extracted and purified from Nostoc flagelliforme under normal, salt stress and mixotrophic culture conditions respectively. Their physicochemical properties and antioxidant activities were investigated. WL-CPS-1, NaCl-CPS-1 and Glu-CPS-1 chemical composition differed in sugar and uronic acid contents, and they were composed of nine constituent monosaccharides and one uronic acid with different ratios, with the average molecular weights of 1.02 × 103, 1.12 × 103 and 1.33 × 103 kDa, respectively. They presented similar fourier transform infrared spectra, but different surface morphology, chain length and branching. Antioxidant assay showed that they all exhibited strong scavenging activity on ABTS+ and hydroxyl radicals and moderate activity on DPPH radical. Glu-CPS-1 exhibited the highest antioxidant activity suggested culture conditions could regulate the bioactivity through influencing the structure and properties. These findings demonstrated the potential application of proper regulation of culture conditions in the development of polysaccharides with high antioxidant activity.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2018.06.111