Prostaglandin D sub(2) induces heme oxygenase-1 in human retinal pigment epithelial cells

The retinal pigment epithelium (RPE) constitutes the blood-retinal barrier, whose function is impaired in various pathological conditions, including cerebral malaria, a lethal complication of Plasmodium falciparum infection. Prostaglandin (PG) D sub(2) is abundantly produced in the brain to regulate...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-03, Vol.367 (2), p.413-419
Main Authors: Kuesap, J, Li, B, Satarug, S, Takeda, K, Numata, I, Na-Bangchang, K, Shibahara, S
Format: Article
Language:English
Subjects:
Plasmodium falciparum
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Summary:The retinal pigment epithelium (RPE) constitutes the blood-retinal barrier, whose function is impaired in various pathological conditions, including cerebral malaria, a lethal complication of Plasmodium falciparum infection. Prostaglandin (PG) D sub(2) is abundantly produced in the brain to regulate sleep responses. Moreover, PGD sub(2) is a potential factor derived from intra-erythrocyte falciparum parasites. Heme oxygenase-1 (HO-1) is important for iron homeostasis via catalysis of heme degradation to release iron, carbon monoxide and biliverdin/bilirubin, and may influence iron supply to the intra-erythrocyte falciparum parasites. Here, we showed that treatment of human RPE cell lines, ARPE-19 and D407, with PGD sub(2) significantly increased the expression levels of HO-1 mRNA, in a dose- and time-dependent manner. Transient expression assays showed that PGD sub(2) treatment increased the HO-1-gene promoter activity through the enhancer sequence, containing a Maf-recognition element. Thus, PGD sub(2) may contribute to the maintenance of heme homeostasis in the brain by inducing HO-1 expression.
ISSN:0006-291X
DOI:10.1016/j.bbrc.2007.12.148