A detection method for endornaviruses from various plant species

Endornaviruses infect hosts in the Kingdoms Plantae, Fungi and Chromista. Conserved genome segments were used to design a set of degenerate primers to detect the presence of endornaviruses in different plant species. The primers were successfully used in preliminary tests, amplifying the predicted D...

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Bibliographic Details
Published in:Phytopathology 2009-06, Vol.99 (6), p.S113-S113
Main Authors: Sabanadzovic, S, Valverde, R A
Format: Article
Language:English
Subjects:
Chromista
Endornavirus
Hordeum vulgare
Oryza sativa
Persea americana
Plantae
Online Access:Get full text
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Summary:Endornaviruses infect hosts in the Kingdoms Plantae, Fungi and Chromista. Conserved genome segments were used to design a set of degenerate primers to detect the presence of endornaviruses in different plant species. The primers were successfully used in preliminary tests, amplifying the predicted DNA segment of 375-384 bp (depending on endornavirus species) from reverse-transcribed dsRNA templates from rice, bean, barley, and avocado. Further work was performed on total RNAs extracted from plant tissue. Primers were also used to detect putative endornaviruses from additional plant hosts and to generate the first data on their genomes. The presence of endornaviruses was confirmed by electrophoretic analyses. Phylogenetic analyses performed on sequences generated in this investigation together with endornavirus data available in the NCBI/GenBank did not distinguished between myco- and plant endornaviruses suggesting their common origin.
ISSN:0031-949X