Capsular Polysaccharide Synthesis Regions in Klebsiella pneumoniae Serotype K57 and a New Capsular Serotype

• Published in: Journal of Clinical Microbiology 2008-07, Vol.46 (7), p.2231-2240
• Main Authors: Pan, Yi-Jiun, Fang, Han-Chi, Yang, Hui-Ching, Lin, Tzu-Lung, Hsieh, Pei-Fang, Tsai, Feng-Chiao, Keynan, Yoav, Wang, Jin-Town
• Format: Article
• Language: English
• Subjects: Bacterial Capsules - genetics; Bacteriology; Biological and medical sciences; Community-Acquired Infections - microbiology; DNA Primers - genetics; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Order; Genes, Bacterial; Genetic Complementation Test; Humans; Immunoblotting; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - classification; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Liver Abscess - microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Multigene Family; Open Reading Frames; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Polysaccharides, Bacterial - classification; Polysaccharides, Bacterial - genetics; Sensitivity and Specificity; Sequence Analysis, DNA; Serotyping; Synteny; Taiwan
• ISSN: 0095-1137; 1098-660X; 1098-5530
• DOI: 10.1128/JCM.01716-07

Community-acquired pyogenic liver abscess caused by Klebsiella pneumoniae is an emerging infectious disease. We explored the capsular polysaccharide synthesis (cps) regions of three non-K1, non-K2 K. pneumoniae strains, A1142, A7754, and A1517, from Taiwanese patients experiencing pyogenic liver abscess. Two of the strains, A1142 and A7754, belonged to capsular serotype K57, while the third belonged to a new capsular serotype, different from the previously reported 77 serotypes. Deletion and complementation experiments suggested that a unique K57 gene, a homologue of wzy, was essential for K57 capsular synthesis and confirmed that this gene cluster was a genetic coding region for K57. Compared to K1 and K2 strains, the three strains were all serum sensitive, suggesting that host factors might also be involved in the three patients. PCR using primers from specific genes for K57 was more sensitive and specific than traditional serotyping. The remaining strain, A1517, did not react to the antisera from any of the 77 serotypes, and none of the 77 reference strains reacted to the serum against this strain. Moreover, PCR analyses using various primer pairs from the serotype-specific open reading frames did not reveal cross-reactivity to any of the 77 reference strains, suggesting that this strain likely represents a new capsular type. We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype.